Showing: 10681 - 10700 of 13976 results
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Plasmid#51571PurposeConstitutively express nuclear localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsNLS and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-VP64
Plasmid#50916PurposeTet-regulatable dCas9-VP64 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and VP64ExpressionMammalianMutationD10A, H840A nuclease-deficientPromoterTREAvailabilityAcademic Institutions and Nonprofits only -
pST1374-N-NLS-flag-linker-Cas9-H840A
Plasmid#51129PurposeCas9 nickase optimized for nuclear import.DepositorInsertCas9 nickase
UseCRISPRTagsFlag and NLSExpressionMammalianMutationH840APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
mEmerald-LaminB1-10
Plasmid#54140PurposeLocalization: Nuclear Envelope, Excitation: 487, Emission: 509DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pSAG1::CAS9-U6::sgUPRT
Plasmid#54467PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.DepositorInsertSAG1 5' UTR
UseCRISPR; Toxoplasma expressionTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pV92.2
Plasmid#78409PurposepKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron constructDepositorInsertsRT-Cas1
Cas2
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_1
Plasmid#78198PurposeExpression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A197C)
UseTagsExpressionBacterialMutationA197C (please see depositors comment below)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
UseTagsExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_3
Plasmid#78200PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,I76G,A197C)
UseTagsExpressionBacterialMutationA17C,I76G,A197C (please see depositor comment bel…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_1
Plasmid#78203PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (G26C)
UseTagsExpressionBacterialMutationG26C (please see depositor comment below)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_2
Plasmid#78204PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (W88C)
UseTagsExpressionBacterialMutationW88C (please see depositor comment below)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pBABE-3xFLAG-OB D166R
Plasmid#79061PurposeRetroviral expression of 3xFLAG tagged, nuclear localized TPP1-OB-RR folding domainDepositorInsertTPP1 (TPP1 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationAspartic acid 166 changed to ArgininePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEF6-myc-TBC1D15
Plasmid#79148Purposemammalian expression of TBC1D15DepositorInsertTBC1D15 (Tbc1d15 Mouse)
UseTagsHis, Myc, and V5ExpressionMammalianMutationS616N (please see depositor comment below)PromoterEF-1alphaAvailabilityAcademic Institutions and Nonprofits only -
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719-2µ-minHIS3
Plasmid#40610DepositorInsertHIS3 (HIS3 Budding Yeast, Synthetic)
UseTagsHAExpressionYeastMutationEvery codon of the coding sequence has been repla…PromoterTDH3AvailabilityAcademic Institutions and Nonprofits only -
pHL1355
Plasmid#37566DepositorInsertsGFP
TetR
CsrA
UseSynthetic BiologyTagsglgC leader regionExpressionBacterialMutationstart codon removedPromoterpConNoHind and pConNoHindM12AvailabilityAcademic Institutions and Nonprofits only -
pHL1530
Plasmid#37569DepositorInsertsGFP
TetR
CsrA
UseSynthetic BiologyTagsglgC leader regionExpressionBacterialMutationstart codon removedPromoterpConNoHind and pLlacO-1AvailabilityAcademic Institutions and Nonprofits only -
pHL1668
Plasmid#37633DepositorInsertsUseSynthetic BiologyTagsASV degradation tag and glgC leader regionExpressionBacterialMutationPromoterpConM12NoHind and pConNoHindAvailabilityAcademic Institutions and Nonprofits only -
pHL1670
Plasmid#37634DepositorInsertsUseSynthetic BiologyTagsASV degradation tag and glgC leader regionExpressionBacterialMutationPromoterpConM12NoHind and pConNoHindAvailabilityAcademic Institutions and Nonprofits only
Showing: 10681 - 10700 of 13976 results