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pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Plasmid

#163076

Purpose

Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Orp1

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HDAC2

Plasmid

#222553

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HDAC2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HDAC2 (HDAC2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-Flag-APEX2-NES

Plasmid

#92158

Purpose

Cytosolic APEX2 construct

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

Flag-APEX2-NES

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A

Plasmid

#24898

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pCFJ1655

Plasmid

#51481

Purpose

miniMos transposable element with HygroR, R4-R3 Gateway cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-Pax6

Plasmid

#32932

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Pax6 (isoform 2, transcript variant 5) (Pax6 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

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POLN_pDEST17

Plasmid

#64885

Purpose

bacterial expression of POLN for purification

Depositor

Richard Wood

Article

Takata et al Nucleic Acids Res. 2010 Jun;38(10):3233-

Insert

POLN (POLN Human)

Use

Tags

Flag and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-GFP

Plasmid

#170176

Purpose

Expresses GFP under the control of EF1a short promoter

Depositor

Xiaojun Lian

Article

Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

July 14, 2024

Availability

Academic Institutions and Nonprofits only

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CAG FUCCI

Plasmid

#175266

Purpose

Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

Depositor

James Monaghan

Article

Duerr et al Development. 2022 Mar 15;149(6). pii: 27

Insert

FUCCI

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ601 - Peft-3 Mos1 transposase

Plasmid

#34874

Purpose

Mos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

Mos1 transpoase

Use

Worm targeting

Tags

Expression

Mutation

Promoter

eef-1A.1 (eft-3)

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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plenti-CAG -IRES-GFP

Plasmid

#69047

Purpose

Lentiviral backbone for GFP coexpression

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only
pMA122 - peel-1 negative selection

Plasmid

#34873

Purpose

Negative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegans

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

peel-1 (peel-1 Nematode)

Use

Worm targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pKH118_keima-YIPF4

Plasmid

#214971

Purpose

Golgiphagy flux reporter keima-YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

keima-YIPF4 (YIPF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-Ms-L-Myc

Plasmid

#26023

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

Insert

L-Myc (Mycl Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
MAC_N_STA5A

Plasmid

#167799

Purpose

Expresses STA5A in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

STA5A (STAT5A Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR1A-NTAP-A (w322-1)

Plasmid

#17402

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame A

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

CBP and SBP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pHAGE TRE dCas9

Plasmid

#50915

Purpose

Tet-regulatable dCas9 lentiviral expression vector

Depositor

Scot Wolfe, Rene Maehr

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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GAL4BD-VP16/pDON207

Plasmid

#201230

Purpose

GAL4-VP16 transcription factor

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

GAL4BD-VP16

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLX301-ZIM3-KRAB-PYL1

Plasmid

#154761

Purpose

Expresses ZIM3 KRAB domain fused to PYL1

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

CMV

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Plasmid

#163059

Purpose

mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Orp1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge