... the Gateway cloning method and its advantages. An introduction to Gateway technology The Gateway cloning...one Gateway vector to produce the desired expression clone. This is possible thanks to the Gateway vectors...Systems (MuLE) Kit, the MultiSite Gateway cloning kit, and MultiSite Gateway plasmids. Constant reading frame...links below to find Gateway plasmids for your organisms of interest: Find Gateway cloning plasmids here... needs. Since its invention in the late 1990s, Gateway cloning technology has become very popular as a...appropriate entry and destination vectors, one can use Gateway to clone a gene of interest into a variety of expression...attB sites creates attL and attR sites.   Gateway vectors contain modified versions of the att sites...

...recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as well as several others... to thoroughly screen colonies for the insert. Gateway® technology (developed by InvitrogenTM) is essentially... a separate post. Focusing on the ccdB aspect, Gateway takes advantage of the same principle that cells...http://teachline.ls.huji.ac.il/72682/tutorials/gateway/introduction.html demonstrating the principal behind...Additional Web Resources OpenWetWare's CcdB Page Gateway Cloning Page at Techline ...focus on a unique gene that makes the popular GatewayTM method possible: ccdB. But what is ccdB, what ...

...generate a wide variety of constructs. Similar, Gateway cloning (discussed below) can run on the order ...more streamlined approach, you might want to give Gateway a try. This method, developed by Invitrogen and..., gel purifications, or ligations. The general Gateway cloning protocol is fairly flexible and adaptable...assembly to make the process close to 100% efficient. Gateway cloning also offers a very high chance of success...inserts to assemble. You could also consider using Gateway if your insert number is a bit smaller, let’s say...

...covered in this segment Hot Plasmid 1: The Tol2 Gateway-Compatible Toolbox for quickly generating zebrafish... diseases. Listen to Our Segement on the Tol 2 Gateway-Compatible Toolbox  Hot Plasmid 2: AAV Vectors ...

...all the latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox for studying the nervous system...system The Cole lab recently deposited the Tol2 Gateway-Compatible Toolobox, which will allow scientists...neurobiology and neuorodgenerative diseases. The Tol2 Gateway-Compatible Toolbox is based on the original Tol2kit...empty vectors that have standard cloning sites or gateway compatible cloning sites for easy cloning of your...toolbox adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst the...2007. PubMed PMID: 17937395 Listen to Our Tol2 Gateway-Compatible Toolbox Podcast Segment The impact of...

...on the Multisite Gateway® technology from Life Technologies. The MultiSite Gateway Kit is a customizable... systems. The MultiSite Gateway Kit contains a set of 12 promoter-less Gateway® entry clones to be used... phage integrase system which is the basis for Gateway cloning. The combination of all 11 att pairs in...customizable Gateway®-based toolkit that allows scientists to generate fusion proteins from any ORF available...as a standard entry clone. ORFs from an ORFeome Gateway library or any PCR-amplified ORF cloned into pDONR221...heterologous promoter outside of the Multisite Gateway® cassette, you can specifically express your new... combined in a specified order using Multisite Gateway cloning. A desired insert is cloned using traditional...

...introduced the “2in1” vector cloning system - a Gateway compatible approach to simultaneously clone two...Grefen and Blatt, 2012). Similar to traditional Gateway™ cloning, the GOI first needs to be cloned into... the GOIs into the 2in1 vector instead of the GatewayTM cloning BP reaction, where insertion of the GOI...

...component plasmids from building blocks via Golden GATEway Cloning Frustrated by the complexity of assembling...combining the advantages of Golden Gate and Multisite Gateway cloning methods developed by the lab of Joachim...just the set of tools that you need. The Golden GATEway cloning kit simplifies the cloning process for ...the destination vectors are used with Multisite Gateway cloning to generate the final construct. Up ...eight entry vectors can be used for each Multisite Gateway compatible destination vector for a maximum of ...transgenesis construct. Plasmids constructed using Golden GATEway cloning have been utilized to create recombination...

...can be assembled with Golden Gate. The popular Gateway cloning system produces constructs with an attB...want to consider using an alternative method like Gateway cloning or Gibson assembly. Another important consideration...

...Bacterial (pET), Gateway GTPase Arl4c 10123 201/192 Gateway GTPase Arl4d 379 201 Gateway GTPase Arl5a 26225...10139 201 Gateway GTPase Sar1a 56681 198 Bacterial (pET), Gateway GTPase Sar1b 51128 198 Gateway GTPase ...9267 398 Gateway GEF Cyth2 9266 400 Mammalian (pEGFP-C1), Gateway GEF Cyth3 9265 399 Gateway GEF Cyth4... 27128 394 Gateway GEF Gbf1 8729 1859 Gateway GEF Psd 5662 1024 GEF Psd2 84249 771 Gateway GEF Psd3 23362...Mammalian (pcDNA3), Gateway GTPase Arf3 377 181 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf4 378...Mammalian (pcDNA3), Gateway GTPase Arf5 381 180 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf6 382...pET), Mammalian (pcDNA3), Gateway GTPase Arl1 400 181 Bacterial (pET), Gateway GTPase Arl2 402 184 Bacterial...

...template vector”. Cloning Method Choose from: Gateway cloning, gene synthesis, Gibson cloning, ligation-independent...For plasmids containing the ccdB gene, such as Gateway vectors, choose the ccdB Survival strain. For plasmids...

...also have more than one function: for example, Gateway cloning relies on genes found on an E. coli fertility...contain this F plasmid is critical for successful Gateway cloning. In addition, scientists have engineered...

...also available as Gateway donor vectors [48218~48221] and can be transferred to Gateway destination vectors...dCas9 Activators Gateway Donors ID Plasmid 48214 pAC1-pCR8-dCas9VP48 : dCas9VP48 on Gateway donor vector ...dCas9 on Gateway donor vector pCR8/GW/TOPO. 48219 pAC147-pCR8-dCas9VP64 : dCas9VP64 on Gateway donor vector...dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. 48221 pAC149-pCR8-dCas9VP160 : dCas9VP160 on Gateway donor vector.../TOPO. Table 4. Gateway Destination Vector ID Plasmid 48222 pAC90-pmax-DEST : Gateway Destination vector... (Link opens in a new window) Lifetechnologies Gateway Destination Vectors ) to adapt to different systems...

...-ccdB - Yeast Gateway expression vector (see more Gateway vectors) TRP1 ...-ccdB - Yeast Gateway expression vector (see more Gateway vectors) LEU2 ...-ccdB - Yeast Gateway expression vector (see more Gateway vectors) HIS3 ...-ccdB - Yeast Gateway expression vector (see more Gateway vectors) Zeocin... experiments Yeast GAL4, PGK, ADH1, ADE2, TRP1 Gateway destination vectors - Inducible...Easy cloning of your gene using Gateway recombination Back to Top Epitope Tag or Fusion... Myc-His tag for mammalian expression (Gateway) SGC plasmids - A variety of His...

...Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can be used for promoter swapping and...PMC4426443. 4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation...

...both Renilla and Firefly luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our... into a Firefly luciferase reporter vector via Gateway cloning to test for the presence of transcriptional...Bartel 101139 pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF) Firefly/Renilla Creation...Insertion of 5' promoter/enhancer regions using Gateway cloning. Lentiviral. Jorge Ferrer 87067 pcDNA3.1...® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2-ccdB-Nanoluc...® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2...® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X...

...Gagoski used SITS to create the pCell-Free vectors, Gateway-compatible backbones that enable cell-free expression...base RNA sequence. The segments are provided in Gateway compatible vectors that allow you to quickly clone...

...location and NOT in the middle of your insert. Gateway cloning utilizes λ integrase to catalyze directional...alternatives to the standard restriction/ligation, Gateway, or Golden Gate cloning methods. Whether it is ...

...plasmids expressing the ccdB gene (important in Gateway cloning). F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15...plasmids expressing the ccdB gene (important in Gateway cloning). F- gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB...

...RESOLUTE Plasmid Collection You may also like... Gateway Cloning Brain Initiative Collection NeuroMab Collection...codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors...and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert...