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PurposeNegative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegans
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 34873 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDESTR4-R3
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Vector typeWorm targeting
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10/P3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepeel-1
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SpeciesC. elegans (nematode)
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Entrez Genepeel-1 (a.k.a. CELE_Y39G10AR.25)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPlease see the publication by the Kruglyak lab for details on the biological role of peel-1: Seidel HS, Ailion M, Li J, van Oudenaarden A, Rockman MV, Kruglyak L. A novel sperm-delivered toxin causes late-stage embryo lethality and transmission ratio distortion in C. elegans. PLoS Biol. 2011 Jul;9(7):e1001115. doi: 10.1371/journal.pbio.1001115.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMA122 - peel-1 negative selection was a gift from Erik Jorgensen (Addgene plasmid # 34873 ; http://n2t.net/addgene:34873 ; RRID:Addgene_34873) -
For your References section:
Improved Mos1-mediated transgenesis in C. elegans. Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM. Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865. 10.1038/nmeth.1865 PubMed 22290181