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Addgene

pCFJ90 - Pmyo-2::mCherry::unc-54utr
(Plasmid #19327)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19327 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 2446
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pmyo-2::mCherry
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2670

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use as co-injection marker. Red mCherry fluorescence in the pharynx. Do not use at high concentration (above 5 ng/ul) as it is toxic to animals.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFJ90 - Pmyo-2::mCherry::unc-54utr was a gift from Erik Jorgensen (Addgene plasmid # 19327 ; http://n2t.net/addgene:19327 ; RRID:Addgene_19327)
  • For your References section:

    Single-copy insertion of transgenes in Caenorhabditis elegans. Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, Blake J Newman, Jason M Thumme, Søren-Peter Olesen, Morten Grunnet & Erik M Jorgensen. 10.1038/ng.248 PubMed 18953339