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Plasmid#123284PurposeLocalization: Outer Mitochondrial MembraneDepositorInsertTOMM20 (TOMM20 Human)
UseTagsHaloTag7ExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDN-Sth1Cas9
Plasmid#221386PurposeCas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attBDepositorInsertSth1 Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN-Cherry-sgRNA
Plasmid#221387Purposeepisomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAsDepositorInsertnone, cloning vector for introduction of targeting sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xHA-hTERT
Plasmid#51637PurposeMammalian expression of 3xHA tagged human TERT, N-terminal.DepositorInsertTERT (TERT Human)
UseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER Short P2A
Plasmid#164108PurposeDetect the short Mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP Mt-ER Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_ATP1.0
Plasmid#167577PurposeExpress the ATP sensor GRAB_ATP1.0 in neuronsDepositorInsertATP sensor GRAB_ATP1.0 (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
DsRed2-Mito-7
Plasmid#55838PurposeLocalization: Mitochondria, Excitation: 558, Emission: 583DepositorInsertMito (COX8A Human)
UseTagsDsRed2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFLAG-GATA2
Plasmid#1418DepositorInsertGATA-2 (GATA2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pH-ePPE
Plasmid#183097PurposeFor plant prime editing in rice plantsDepositorInsertNls-nCas9-NC-nls-MLV-Nls
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9PromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1179 U6-reci Gag-Cas9 v2
Plasmid#201914PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-Cas9 v2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES_Tomato
Plasmid#107229PurposeTomato expressing plasmidDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40
Plasmid#105540PurposeAAV expression of EGFP-Cre from hSyn promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV5, AAV8, and AAV9InsertEGFP-Cre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhSynAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep
Plasmid#92099PurposeDoyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.DepositorTypeEmpty backboneUseCRISPR and TALEN; ZfnTagsExpressionMammalianMutationPromoterTet-On 3G BidirectionalAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mito-B
Plasmid#209864PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVSDepositorInsertddGFP B
UseTagsTargeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV14-3X-Flag-SARS-CoV-2 S
Plasmid#145780PurposeOver expression of SARS-CoV-2 S (spike) in mammalian cell lineDepositorInsertSARS-CoV-2 S (S SARS CoV 2)
UseTags3xFlagExpressionMammalianMutationCodon optimized and C-terminal 19 amino acids del…PromoterCMVAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
mcherry-DN KASH
Plasmid#125553Purposemcherry labeled DN-KASHDepositorInsertSYNE1 (SYNE1 Human)
UseTagsmcherryExpressionMammalianMutationTruncated form: only the KASH domain of nesprin-…PromoterCMVAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
TurboID-His6_pET21a
Plasmid#107177Purposeexpresses His6-tagged TurboID in bacteriaDepositorInsertTurboID (BirA mutant)
UseTagsExpressionBacterialMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRia-v2
Plasmid#84832PurposeCRISPRi/a V2 library parental plasmidDepositorInsertssgRNA
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone
Plasmid#216152PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11']DepositorInsertmScarlet with cryptic exon and C-terminal FLAG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas13d-dsRBD
Plasmid#154938PurposeBPNLS-dRfxCas13d-dsRBD-BPNLS-HADepositorInsertBPNLS-dRfxCas13d-dsRBD-BPNLS-HA
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
secreted BirA-Flag
Plasmid#64395PurposeFlag-tagged biotinylation enzyme for co-expression with bio-proteinsDepositorInsertBirA (birA E. coli)
UseTagsFlag tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBEN276
Plasmid#69150PurposeIntegration of luciferase operon in Tn7 insertion site of Gram negative bacteriaDepositorInsertluxCDABE
UseLuciferaseTagsExpressionMutationPromoterfrrAvailable sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
PCV-Cas9
Plasmid#123643PurposeExpresses Cas9 with an N-terminal HUH-tag called PCV2DepositorInsertsUseTags(EAAAK)8 linker and His6 + SUMOExpressionBacterialMutation2-116PromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF3036
Plasmid#144512PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertATF4 (ATF4 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pDisplay-iSeroSnFR
Plasmid#128484PurposeFluorescent reporter for serotonin (mammalian expression, membrane localization tag)DepositorInsertiSeroSnFR
UseTagsIg-kappa leader, Myc, PDGFR + enhanced ER export,…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
MP9956:SFG.GD3synthase-2A-GD2synthase
Plasmid#75013PurposeRetroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.DepositorInsertsmurine GD3 synthase
murine GD2 synthase
UseRetroviral; Momlv retroviral vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21b(+)-Is-PETase
Plasmid#112202PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
UseTags6XHISExpressionBacterialMutationPromoterN/AAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TBG-ER-TurboID
Plasmid#149415PurposeHepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorInsertER-TurboID
UseAAVTagsHA and V5ExpressionMutationPromoterTBGAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHSP02
Plasmid#117259PurposeGenome editing for Lactobacillus plantarum WCSF1DepositorInsertCas9, promotor P11-guide-RNA, homologous arms of Lp_0537
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW empty vector
Plasmid#184708PurposeLentiviral empty vector for doxycycline-inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BRCA1
Plasmid#119007PurposeMammalian expression of human BRCA1 and BFPDepositorInsertBRCA1 (BRCA1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2497
Plasmid#142115PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertASCL1 (ASCL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-Ec1
Plasmid#138474PurposeEnables high-efficiency recombineering in E. coliDepositorInsertsCspRecT
mutL E32K
UseTagsExpressionBacterialMutationE32KPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DEST47 PINK1 C-GFP
Plasmid#13316DepositorInsertPINK1 (PINK1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRL-SV40P
Plasmid#27163PurposeRenilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experimentsDepositorInsertSV40 promoter
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE1
Plasmid#90335PurposeNFkB - p50/p65 pathway gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterNFKBAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-R-GECO1
Plasmid#46021PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertR-GECO1
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone
Plasmid#216154PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5']DepositorInsertmScarlet with cryptic exon and C-terminal FLAG
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only