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Addgene

His6-TEV-CRBN-midi
(Plasmid #215330)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215330 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pNIC28-Bsa4
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 7280
  • Total vector size (bp) 8318
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21 (DE3) for bacterial expression of encoding protein
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CRBN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1038
  • Mutation
    contains amino acids 41-187 followed by a Gly-Ser-Gly linker and amino acids 249-426, with mutations C78I, I92V, K116N, Q134E, R283W, C287N, V293S, G302D, L342R, C343E, T359I and L423I
  • GenBank ID
    NM_016302.4
  • Entrez Gene
    CRBN (a.k.a. MRT2, MRT2A)
  • Promoter lacI
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.01.17.575503 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His6-TEV-CRBN-midi was a gift from Alessio Ciulli (Addgene plasmid # 215330 ; http://n2t.net/addgene:215330 ; RRID:Addgene_215330)
  • For your References section:

    Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders. Kroupova A, Spiteri VA, Rutter ZJ, Furihata H, Darren D, Ramachandran S, Chakraborti S, Haubrich K, Pethe J, Gonzales D, Wijaya AJ, Rodriguez-Rios M, Sturbaut M, Lynch DM, Farnaby W, Nakasone MA, Zollman D, Ciulli A. Nat Commun. 2024 Oct 15;15(1):8885. doi: 10.1038/s41467-024-52871-9. 10.1038/s41467-024-52871-9 PubMed 39406745