pIVM_26
(Plasmid #204501)

• Purpose: Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)
• Depositing Lab: Alessio Ciulli
• Publication: Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3. doi: 10.1016/j.str.2017.04.009.

Backbone

• Vector backbone: pCDF-1b DUET
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3781
• Total vector size (bp): 4540
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21 or Rosetta and drop temperature to 23°C for expression of protein
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EloB (1-104) and EloC (17-112)
• Species: H. sapiens (human)
• Insert Size (bp): 757
• Mutation: 0
• GenBank ID: NP_009039.1 (EloB) NP_001191792 (EloC)
• Entrez Gene: ELOC (a.k.a. SIII, TCEB1)
• Entrez Gene: ELOB (a.k.a. SIII, TCEB2)
• Promoter: AmpR

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nco1 (EloB) and Nde1 (EloC) (not destroyed)
• 3′ cloning site: HindIII (EloB) and Xho1 (EloC) (not destroyed)
• 5′ sequencing primer: DUET primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gadd MS, Testa A, Lucas X, Chan KH, Chen W, Lamont DJ, Zengerle M, Ciulli A. Structural basis of PROTAC cooperative recognition for selective protein degradation. Nat Chem Biol. 2017 May;13(5):514-521. doi: 10.1038/nchembio.2329. Epub 2017 Mar 13. PMID: 28288108; PMCID: PMC5392356.