Cas12j-8-puro-crRNA
(Plasmid
#194965)
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Purpose(Empty Backbone) The plasmid expresses human codon-optimized Cas12j-8, crRNA expression elements and puromycin resistence gene.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV2 ITR vector
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cas12j-8-puro-crRNA was a gift from Yongming Wang (Addgene plasmid # 194965 ; http://n2t.net/addgene:194965 ; RRID:Addgene_194965) -
For your References section:
A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing. Wang Y, Qi T, Liu J, Yang Y, Wang Z, Wang Y, Wang T, Li M, Li M, Lu D, Chang ACY, Yang L, Gao S, Wang Y, Lan F. Sci Adv. 2023 Feb 10;9(6):eabo6405. doi: 10.1126/sciadv.abo6405. Epub 2023 Feb 10. 10.1126/sciadv.abo6405 PubMed 36763662