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pCDNA3.1-NKD2S31A

Plasmid

#174331

Purpose

encodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Use

Tags

MycHis

Expression

Mammalian

Mutation

changed Serine 31 to Alanine

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nl

Plasmid

#172463

Purpose

expresses wild-type protein when injected into embryos

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nl (wbp2nl.L Frog)

Use

Xenopus expression

Tags

none

Expression

Mutation

none

Promoter

SP6

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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UUUUA-pHG165c

Plasmid

#170107

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the UreR urea ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

UUUUA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MMMMA-pHG165c

Plasmid

#170108

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the MelR L-melibiose ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

MelR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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XXXXA-pHG165c

Plasmid

#170110

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

XXXXA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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XXXXA_Q182A_R287H-pHG165c

Plasmid

#170111

Purpose

Constitutively expressed , mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

XXXXA_Q182A_R278H

Use

Tags

Expression

Bacterial

Mutation

Q182A, R287H

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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RRRAA-pHG165c

Plasmid

#170112

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

RRRAA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDY153: Flag(SUZ12) WT HDR plasmid

Plasmid

#170793

Purpose

WT homology directed repair plasmid for performing genome-editing on the SUZ12 Locus

Depositor

Thomas Cech

Article

Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

Insert

SUZ12 (SUZ12 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT I261A

Plasmid

#153476

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT I261A

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, I261A

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT S333A

Plasmid

#153477

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT S333A

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, S333A

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin-TAPBPR 22-35

Plasmid

#153478

Purpose

Mammalian expression of FLAG-tagged Tapasin with TAPBPR a.a. 22-35

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Tapasin a.a.10-20 replaced with TAPBPR a.a. 22-35

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin delta2step

Plasmid

#153479

Purpose

Mammalian expression of FLAG-tagged Tapasin delta2step

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

deletion of aa.13-14, 16-17

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only
pCEP4-FLAG-Tapasin delta3step

Plasmid

#153480

Purpose

Mammalian expression of FLAG-tagged Tapasin delta3step

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

deletion of aa.12-14, 16-18

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only
pCEP4-FLAG-Tapasin G15E

Plasmid

#153481

Purpose

Mammalian expression of FLAG-tagged Tapasin G15E

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

G15E

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin G15L

Plasmid

#153482

Purpose

Mammalian expression of FLAG-tagged Tapasin G15L

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

G15L

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin L18E

Plasmid

#153483

Purpose

Mammalian expression of FLAG-tagged Tapasin L18E

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

L18E

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-Tapasin L18G

Plasmid

#153484

Purpose

Mammalian expression of FLAG-tagged Tapasin L18G

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

L18G

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-FLAG-Tapasin L18K

Plasmid

#153485

Purpose

Mammalian expression of FLAG-tagged Tapasin L18K

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBP (TAPBP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

L18K

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pETCON-Aga2p-TAPBPR-myc

Plasmid

#153486

Purpose

Yeast expression of Aga2p-TAPBPR-myc

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Use

Tags

Aga2P and c-myc epitope tag

Expression

Yeast

Mutation

Promoter

GAL

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT

Plasmid

#141309

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge