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pETCON-Aga2p-TAPBPR-myc
(Plasmid #153486)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153486 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETCON
  • Backbone size w/o insert (bp) 6287
  • Total vector size (bp) 7439
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TAPBPR
  • Alt name
    TAPBPL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1152
  • GenBank ID
    BC015017
  • Entrez Gene
    TAPBPL (a.k.a. TAPBP-R, TAPBPR)
  • Promoter GAL
  • Tags / Fusion Proteins
    • Aga2P (N terminal on insert)
    • c-myc epitope tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer None
  • 3′ sequencing primer cgagctaaaagtacagtggg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETCON-Aga2p-TAPBPR-myc was a gift from Erik Procko (Addgene plasmid # 153486 ; http://n2t.net/addgene:153486 ; RRID:Addgene_153486)

  • For your References section:

    TAPBPR promotes antigen loading on MHC-I molecules using a peptide trap. McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, Procko E, Sgourakis NG. Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6. 10.1038/s41467-021-23225-6 PubMed 34039964
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