pCEP4-FLAG-TAPBPR-tapasinCT I261A
(Plasmid
#153476)
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PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT I261A
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153476 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCEP4
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 10381
- Total vector size (bp) 11965
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTAPBPR
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SpeciesH. sapiens (human)
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Insert Size (bp)1584
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MutationC terminal tail of tapasin, I261A
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Entrez GeneTAPBPL (a.k.a. TAPBP-R, TAPBPR)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV_fwd
- 3′ sequencing primer EBV_rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCEP4-FLAG-TAPBPR-tapasinCT I261A was a gift from Erik Procko (Addgene plasmid # 153476 ; http://n2t.net/addgene:153476 ; RRID:Addgene_153476) -
For your References section:
TAPBPR promotes antigen loading on MHC-I molecules using a peptide trap. McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, Procko E, Sgourakis NG. Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6. 10.1038/s41467-021-23225-6 PubMed 34039964