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pMCS.71/2272

Plasmid

#22732

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Feb 14. ():.

Insert

Lox71-MCS-Lox2272

Use

Cre/Lox

Tags

Expression

Mutation

This is a basal exchange vector for RMCE containi…

Promoter

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

Plasmid

#108769

Purpose

Expresses N-terminus to aa396 of Flp recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa396

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB2227

Plasmid

#67536

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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Col2a1:TFPnls-mErt-Cre-Ert

Plasmid

#111152

Purpose

Chondrocyte specific inducible Cre

Depositor

Elly Tanaka

Insert

TFPnls-T2a-mErt-Cre-Ert

Use

Cre/Lox

Tags

Expression

Mutation

wt

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-100RPU.2-coEPO-lox2272

Plasmid

#154828

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietin

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

coEPO

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Codon-optimized (Cricetulus griseus)

Promoter

100RPU.2

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p2lox-N-(His-6X)-cAMPr

Plasmid

#102439

Purpose

cAMPr with a directly fused N-terminal 6x His tag

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr

Use

Cre/Lox

Tags

6x His

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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TRE.IkBSR-Flag Pgk.Cre

Plasmid

#22504

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

IkBSR

Use

Cre/Lox and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pH3FS-GAL1

Plasmid

#85782

Purpose

N-ICE plasmid pH3FS-GAL1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pWPT-nlsLacZ

Plasmid

#12261

Depositor

Didier Trono

Insert

LacZ

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pSicoR human DGCR8-2

Plasmid

#14770

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

DGCR8 shRNA (DGCR8 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-Con/Foff 2.0-sRGECO

Plasmid

#137127

Purpose

Intersectional viral expression of sRGECO in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

E217D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CA-FLEX-RG

Plasmid

#38043

Depositor

Naoshige Uchida

Article

Watabe-Uchida et al Neuron. 2012 Jun 7;74(5):858-73.

Insert

Rabies virus glycoprotein

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CA

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pBigTB-FlpERT2

Plasmid

#149435

Purpose

tamoxifen-inducible Flp recombinase

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Flp-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS)

Plasmid

#137864

Purpose

Monomeric TagBFP2 and iCre-ERT2 recombinase (PAGSAS N-terminus)

Depositor

Mario Capecchi

Insert

mTagBFP2-T2A-iCreERT2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pREPROII-attB

Plasmid

#86557

Purpose

Dox inducible expression of reprogramming factors (Klf4, Sox2, Oct4, & cMyc), integration via phiC31 attB sites, excision via Cre/loxP

Depositor

Ralf Kuehn

Article

Merkl et al PLoS One. 2013;8(1):e55170. doi: 10.1371

Inserts

Oct4 (Pou5f1 Mouse)
cMyc (Myc Mouse)
Sox2 (Sox2 Mouse)
Kruppel-like factor 4 (Klf4 Mouse)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP

Plasmid

#137150

Purpose

Intersectional viral expression of Arch3.3-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-Arch3.3-p2a-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_SynP_DIO_tAKARα- T391A_WPRE

Plasmid

#196676

Purpose

AAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).

Depositor

Haining Zhong

Article

Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

Insert

tAKARα-T391A

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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Ai170 (TIT2L-ASAP2s-Kv-ICL-tTA2) targeting vector

Plasmid

#114435

Purpose

Target a Cre-dependent voltage sensor ASAP2s cassette into the mouse TIGRE locus

Depositor

Michael Lin, Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

ASAP2s-Kv, tTA2

Use

Cre/Lox and Mouse Targeting

Tags

Kv2.1 tag

Expression

Mutation

Promoter

TRE2, CAG

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFPpA-ACN

Plasmid

#20343

Depositor

Mario Capecchi

Article

Wu et al Nat Protoc. 2008 . 3(6):1056-76.

Insert

EGFPpA-ACN

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

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R26-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

Plasmid

#180151

Purpose

td-sfGFP cre reporter with Rosa26 recombineering homology arms

Depositor

Mario Capecchi

Insert

td-sfGFP

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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