pREPROII-attB
(Plasmid
#86557)
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PurposeDox inducible expression of reprogramming factors (Klf4, Sox2, Oct4, & cMyc), integration via phiC31 attB sites, excision via Cre/loxP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86557 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBluescript
- Backbone size w/o insert (bp) 2770
- Total vector size (bp) 14670
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Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameOct4
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SpeciesM. musculus (mouse)
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Insert Size (bp)1056
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Entrez GenePou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer TRE-F (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namecMyc
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SpeciesM. musculus (mouse)
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Entrez GeneMyc (a.k.a. Myc2, Niard, Nird, bHLHe39)
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert nameSox2
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SpeciesM. musculus (mouse)
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Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
Cloning Information for Gene/Insert 3
- Cloning method Gibson Cloning
- 5′ sequencing primer TRE-R (Common Sequencing Primers)
Gene/Insert 4
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Gene/Insert nameKruppel-like factor 4
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Alt nameKruppel-like factor 4
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SpeciesM. musculus (mouse)
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Entrez GeneKlf4 (a.k.a. EZF, Gklf, Zie)
Cloning Information for Gene/Insert 4
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pREPROII-attB was a gift from Ralf Kuehn (Addgene plasmid # 86557 ; http://n2t.net/addgene:86557 ; RRID:Addgene_86557) -
For your References section:
Efficient generation of rat induced pluripotent stem cells using a non-viral inducible vector. Merkl C, Saalfrank A, Riesen N, Kuhn R, Pertek A, Eser S, Hardt MS, Kind A, Saur D, Wurst W, Iglesias A, Schnieke A. PLoS One. 2013;8(1):e55170. doi: 10.1371/journal.pone.0055170. Epub 2013 Jan 31. 10.1371/journal.pone.0055170 PubMed 23383095