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PurposepiggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for creation of induced pluripotent stem cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20959 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePB-TET
- Backbone size w/o insert (bp) 9186
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Vector typeMammalian Expression ; piggyBac
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecMyc, KLF4, Oct4, Sox2
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SpeciesM. musculus (mouse)
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Insert Size (bp)5046
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Entrez GeneMyc (a.k.a. Myc2, Niard, Nird, bHLHe39)
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Entrez GeneKlf4 (a.k.a. EZF, Gklf, Zie)
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Entrez GenePou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
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Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
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Tag
/ Fusion Protein
- IRES-bGeo (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site attB1 (not destroyed)
- 3′ cloning site attB2 (not destroyed)
- 5′ sequencing primer M13 F, T7
- 3′ sequencing primer M13 R, T3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMKOS liked by 2A peptides obtained from Keisuke Kaji et al (Nature. 2009 Mar 1) piggyBac terminal repeats obtained from Pentao Liu at Sanger.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional reference, Kaji et al Nature. 2009 Mar 1
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-TET-MKOS was a gift from Andras Nagy (Addgene plasmid # 20959 ; http://n2t.net/addgene:20959 ; RRID:Addgene_20959) -
For your References section:
piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hämäläinen R, Cowling R, Wang W, Liu P, Gertsenstein M, Kaji K, Sung HK, Nagy A. Nature. 2009 Mar 1. ():. 10.1038/nature07863 PubMed 19252478