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Showing: 121 - 160 of 1215 results

  1. PB-TET

    Plasmid
    #20909
    Purpose
    piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)
    Depositor
    Andras Nagy
    Article
    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi
    Insert
    Gateway Destination Vector
    Use
    piggybac
    Tags
    IRES-bGeo
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2009
    Availability
    Academic Institutions and Nonprofits only
    20909-plasmid-map-sequence-id-174009

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  3. PB-eDIO-ChR2-EYFP

    Plasmid
    #104387
    Purpose
    PiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.
    Depositor
    Volker Busskamp
    Article
    Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10
    Insert
    Channelrhodopsin 2
    Use
    Cre/Lox; Piggybac
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    E134R
    Promoter
    Available since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    104387-plasmid-map-sequence-id-203589

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  6. SV40-MCS-Z1

    Plasmid
    #127639
    Purpose
    Empty backbone plasmid with SV40 promoter and SV40 polyA with Zeocin/Bleocin bacterial resistance
    Depositor
    Jordan Green
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40 promoter
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127639-plasmid-map-sequence-id-273360

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  7. PB ID1-iCreERT2

    Plasmid
    #139280
    Purpose
    Human ID1-iCreERT2 transgene piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    ID1 (ID1 Human)
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Replaced the ID1 protein coding sequence in the t…
    Promoter
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    139280-plasmid-map-sequence-id-315091

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  8. pScarlessHD-DsRed

    Plasmid
    #64703
    Purpose
    Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    piggyBac-3xP3-DsRed
    Use
    Tags
    Expression
    Mutation
    Promoter
    3xP3
    Available since
    Dec. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    64703-plasmid-map-sequence-id-443894

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  14. pDEST-PB-R4R3

    Plasmid
    #139518
    Purpose
    Provides two PiggyBac transposon sequences between gene cassettes in the destination vector
    Depositor
    Minoru Ko
    Type
    Empty backbone
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139518-plasmid-map-sequence-id-274611

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  16. SV40-eGFP-Z1

    Plasmid
    #127638
    Purpose
    Minimal backbone plasmid for mammalian eGFP expression from SV40 promoter with zeocin/bleocin bacterial resistance
    Depositor
    Jordan Green
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127638-plasmid-map-sequence-id-248430

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  17. PB-PEmax

    Plasmid
    #187893
    Purpose
    All-in-one prime editor piggyBac transposon with PEmax
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    BPSV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    187893-plasmid-map-sequence-id-370734

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  18. PB-PEmax NG

    Plasmid
    #187894
    Purpose
    All-in-one prime editor piggyBac transposon with PEmax, NG variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    BPSV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    187894-plasmid-map-sequence-id-370740

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  19. PB-PE xCas9

    Plasmid
    #172652
    Purpose
    All-in-one prime editor piggyBac transposon (xCas9)
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    172652-plasmid-map-sequence-id-346754

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  20. PB-PEmax SpG

    Plasmid
    #187895
    Purpose
    All-in-one prime editor piggyBac transposon with PEmax, SpG variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    BPSV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    187895-plasmid-map-sequence-id-370756

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  21. PB-PE SpRY

    Plasmid
    #179082
    Purpose
    All-in-one prime editor piggyBac transposon, SpRY variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…
    Promoter
    CAG
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179082-plasmid-map-sequence-id-352656

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  22. PB-PE SpG

    Plasmid
    #179081
    Purpose
    All-in-one prime editor piggyBac transposon, SpG variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R
    Promoter
    CAG
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179081-plasmid-map-sequence-id-352652

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  23. PB-PE VQR

    Plasmid
    #162797
    Purpose
    All-in-one prime editor piggyBac transposon, VQR variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    CAG
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    162797-plasmid-map-sequence-id-320036

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  24. PB-PE NG

    Plasmid
    #174554
    Purpose
    All-in-one prime editor piggyBac transposon, NG variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    L1111R, G1218R, E1219F, A1322R, R1335V, T1337R
    Promoter
    CAG
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    174554-plasmid-map-sequence-id-354966

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  25. PB-TET-PH-lox66FRT

    Plasmid
    #139561
    Purpose
    piggyBac expression vector with TET-ON system and puromycin selection
    Depositor
    Minoru Ko
    Insert
    PGK-puroR-pA cassette, inserted in PB-TET vector
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139561-plasmid-map-sequence-id-274608

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  26. PB-TET-ZH-lox66FRT

    Plasmid
    #139563
    Purpose
    piggyBac expression vector with TET-ON system and Zeocin selection
    Depositor
    Minoru Ko
    Insert
    PGK-ZeoR-pA cassette, inserted in PB-TET vector
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139563-plasmid-map-sequence-id-274610

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  27. PB-TET-BH-lox66FRT

    Plasmid
    #139562
    Purpose
    piggyBac expression vector with TET-ON system and Blasticidin selection
    Depositor
    Minoru Ko
    Insert
    PGK-BSD-pA cassette, inserted in PB-TET vector
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139562-plasmid-map-sequence-id-274605

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  29. pScarlessHD-sfGFP-DsRed

    Plasmid
    #80811
    Purpose
    Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    sfGFP-piggyBac-DsRed
    Use
    Unspecified
    Tags
    sfGFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80811-plasmid-map-sequence-id-152800

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  30. pScarlessHD-3xFLAG-DsRed

    Plasmid
    #80820
    Purpose
    Vector containing a cassette for scarless insertion of 3xFLAG and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    3xFLAG-piggyBac-DsRed
    Use
    Unspecified
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80820-plasmid-map-sequence-id-152798

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  31. pScarlessHD-2xHA-DsRed

    Plasmid
    #80822
    Purpose
    Vector containing a cassette for scarless insertion of 2xHA and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    2xHA-piggyBac-DsRed
    Use
    Unspecified
    Tags
    2xHA
    Expression
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80822-plasmid-map-sequence-id-152787

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  34. PB Neo* PB

    Plasmid
    #138413
    Purpose
    Minimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Piggybac
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138413-plasmid-map-sequence-id-316185

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  35. pScarlessHD-DsRed-w+

    Plasmid
    #80801
    Purpose
    Vector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Inserts
    piggyBac-3xP3-DsRed
    GMR-white
    Use
    High copy, amp resistance
    Tags
    Expression
    Mutation
    Promoter
    3xP3 and GMR::hsp70
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80801-plasmid-map-sequence-id-152801

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  39. pB PUb-integrase

    Plasmid
    #183966
    Purpose
    piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker
    Depositor
    Eric Marois
    Article
    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:
    Insert
    PhiC31 integrase
    Use
    Piggybac insect transgenesis destination vector
    Tags
    Expression
    Mutation
    Promoter
    Aedes aegypti Polyubiquitin
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183966-plasmid-map-sequence-id-361316

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Showing: 121 - 160 of 1215 results