-
PurposepiggyBac vector with tet inducible mSox2
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 20908 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonePB-TET
- Backbone size w/o insert (bp) 9186
-
Vector typeMammalian Expression ; piggyBac
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSox2
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)960
-
Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
-
Tag
/ Fusion Protein
- IRES-bGeo (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attB1 (not destroyed)
- 3′ cloning site attB2 (not destroyed)
- 5′ sequencing primer M13 F, T7
- 3′ sequencing primer M13 R, T3 (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made bySox2 gateway cloned from Yamanaka Plasmid 13367: pMXs-Sox2, piggyBac terminal repeats obtained from Pentao Liu at Sanger.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PB-TET-mSox2 was a gift from Andras Nagy (Addgene plasmid # 20908 ; http://n2t.net/addgene:20908 ; RRID:Addgene_20908) -
For your References section:
piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hämäläinen R, Cowling R, Wang W, Liu P, Gertsenstein M, Kaji K, Sung HK, Nagy A. Nature. 2009 Mar 1. ():. 10.1038/nature07863 PubMed 19252478