...plasmids with the piggyBac™ transposon. These plasmids, when combined with a source of piggyBac™ transposase...Goodwin from Transposagen What is the piggyBac™ transposon? The piggyBac™ transposon was originally identified... transposons, piggyBac™ has two components, a transposon and a transposase. The piggyBac™ transposase ...excision-only piggyBac™ transposase. Image courtesy of Transposagen.   How does piggyBac™ enhance gene...technology with the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon ...removed using the piggyBac™ transposase. This can be best achieved by utilizing a piggyBac™ transposon in...re-integration from the piggyBac™ donor, which is typically found with the wild-type piggyBac™ transposase enzyme...

...Sleeping Beauty plasmids  piggyBac Although its name suggests otherwise, piggyBac was discovered in the cabbage...compared to the codon-optimized wild-type piggyBac transposase.  piggyBac plasmids    Tol2  Tol2 was the first...adapted for use as research tools: Sleeping beauty, PiggyBac, and Tol2.  Sleeping beauty Sleeping Beauty is...behind a DNA footprint sequence after excision. piggyBac can mobilize DNA over 100 kB in size and is active...insect, and mammalian cells, including human cells. piggyBac is biased to integrate at transcription start ... hypersensitivity sites. Like Sleeping Beauty, piggyBac has an integration efficiency in human cells similar...Koga et al., 1996). Unlike Sleeping Beauty and piggyBac, Tol2 has a weak consensus sequence for its preferred...

...Broad Institute developed fluorescently labeled piggyBac vectors for uniform and sustained expression of... by a doxycycline inducible promoter. The gRNA piggyBac plasmids include a mRFP reporter. With this system...expressing both dCas9 and gRNAs. The multi-gRNA piggyBac vector integrates into genomic DNA with the help...transcript and protein levels. Plasmids for multiplexed piggyBac gRNA delivery and the all-in-one dCas9-KRAB and...found at Addgene. Check out these plasmids for piggyBac gRNA delivery and the all-in-one dCas9 targeting...

...mARG plasmids Our new mARGs come as a set of two PiggyBac integratable cassettes, both with doxycycline ... cotransfecting them with a plasmid expressing PiggyBac transposase. In rtTA-cell lines, it is better ...line with rtTA, then integrate the mARGs using PiggyBac. Gas vesicles are detectable under ultrasound ...cotransfect the mARG cassettes 2:1 (A:N-V) with PiggyBac transposase and outgrow the cells for a several...

... Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system for human pluripotent stem cells...Barrett’s lab developed new fluorescently labeled piggyBac vectors to deliver uniform and sustained expression...

...Insertional mutagenesis, residual expression piggyBac Excisible Medium Medium Generates transgene-free... considered safe for therapeutic applications. piggyBac (PB) transposon: The PB transposon is a mobile...transgene-free induced pluripotent mouse stem cells by the piggyBac transposon." Nature Methods 6, no. 5 (2009): 363...

...No Tilmann Weber pPBT-peRNA_GG-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen...Mikkelsen pPBT-PE2-PuroTK-pegRNA_GG Mammalian, piggyBac hU6 pegRNA BsmBI Yes (Cas9 H840A + MMLV RT) PuroTK...

...25. 126(4):663-76. Yamanaka piggyBac Mouse Doxycycline-inducible piggyBac transposon reprogramming system...2011 Mar;21(3):518-29. Cheng piggyBac Mouse Doxycycline-inducible piggyBac transposon reprogramming system... Klf4, and c-Myc from polycistronic cassettes. piggyBac transposition reprograms fibroblasts to induced...

...mutants (Wen et al., 2018). SRIRACCHA uses the Piggybac transposon to stably integrate a reporter gene...

... out Addgene's Lentiviral dCas9 SAM vector and Piggybac dCas9 SAM vector! On/off control all the time ...

...from AAV2) Mammalian Transposon pRDA_757 (Piggybac; constitutive expression) Mammalian Plasmid...

... CRISPR Competitor NgAgo Learn How You Can Use piggyBac Vectors in Genome Editing Resources on Addgene.org...

...lead to comparable experimental results. Using PiggyBac transposons, the Woltjen Lab compared different...reprogramming discrepancies. The polycistronic piggyBac transposon vectors tested in this work are available...

...uses the piggybac transposase to integrate dCas9-VPR into the genome of target cells. The piggybac system...McMahon A, et al. Cell. 2016. PubMed PMID: 27040499 PiggyBac mediated delivery of robust CRISPR activators ...

... toolbox . Lam et al. bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription...

...CMV Spinocerebellar Ataxia Richard Youle 176485 Piggybac-TetO-hNurr1-blast NR4A2 TetO Parkinson's Marius...ApoE4 APOE CMV Alzheimer's Bradley Hyman 197908 piggybac-EF1-halo-mTBP TBP Halo EF1a Parkinson's James ...GST-TEV-TBK1 TBK1 polH ALS Sascha Martens 209078 piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (...SNCA GFP TRE Parkinson's Vikram Khurana 209080 piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro...SNCA GFP TRE Parkinson's Vikram Khurana 209081 piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2...SNCA Avi CMV Parkinson's Vikram Khurana 209085 piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA... SNCA TRE Parkinson's Vikram Khurana 209086 piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA...

...enzymes. CRISPR Transposases Transposon systems like piggyBac or Tn7 allow for large scale edits by integrating...

...enzymes. CRISPR Transposases Transposon systems like piggyBac or Tn7 allow for large scale edits by integrating...