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Showing: 1 - 12 of 12 results
  1. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...plasmids with the piggyBac™ transposon. These plasmids, when combined with a source of piggyBac™ transposase...Goodwin from Transposagen What is the piggyBac™ transposon? The piggyBac™ transposon was originally identified... transposons, piggyBac™ has two components, a transposon and a transposase. The piggyBac™ transposase ...excision-only piggyBac™ transposase. Image courtesy of Transposagen.   How does piggyBac™ enhance gene...technology with the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon ...removed using the piggyBac™ transposase. This can be best achieved by utilizing a piggyBac™ transposon in...re-integration from the piggyBac™ donor, which is typically found with the wild-type piggyBac™ transposase enzyme...
  2. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...Sleeping Beauty plasmids  piggyBac Although its name suggests otherwise, piggyBac was discovered in the cabbage...compared to the codon-optimized wild-type piggyBac transposase.  piggyBac plasmids    Tol2  Tol2 was the first...adapted for use as research tools: Sleeping beauty, PiggyBac, and Tol2.  Sleeping beauty Sleeping Beauty is...behind a DNA footprint sequence after excision. piggyBac can mobilize DNA over 100 kB in size and is active...insect, and mammalian cells, including human cells. piggyBac is biased to integrate at transcription start ... hypersensitivity sites. Like Sleeping Beauty, piggyBac has an integration efficiency in human cells similar...Koga et al., 1996). Unlike Sleeping Beauty and piggyBac, Tol2 has a weak consensus sequence for its preferred...
  3. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...Broad Institute developed fluorescently labeled piggyBac vectors for uniform and sustained expression of... by a doxycycline inducible promoter. The gRNA piggyBac plasmids include a mRFP reporter. With this system...expressing both dCas9 and gRNAs. The multi-gRNA piggyBac vector integrates into genomic DNA with the help...transcript and protein levels. Plasmids for multiplexed piggyBac gRNA delivery and the all-in-one dCas9-KRAB and...found at Addgene. Check out these plasmids for piggyBac gRNA delivery and the all-in-one dCas9 targeting...
  4. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...mARG plasmids Our new mARGs come as a set of two PiggyBac integratable cassettes, both with doxycycline ... cotransfecting them with a plasmid expressing PiggyBac transposase. In rtTA-cell lines, it is better ...line with rtTA, then integrate the mARGs using PiggyBac. Gas vesicles are detectable under ultrasound ...cotransfect the mARG cassettes 2:1 (A:N-V) with PiggyBac transposase and outgrow the cells for a several...
  5. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ... Find the CRISPRi plasmids A multiplexed gRNA piggyBac transposon system for human pluripotent stem cells...Barrett’s lab developed new fluorescently labeled piggyBac vectors to deliver uniform and sustained expression...
  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...Insertional mutagenesis, residual expression piggyBac Excisible Medium Medium Generates transgene-free... considered safe for therapeutic applications. piggyBac (PB) transposon: The PB transposon is a mobile...transgene-free induced pluripotent mouse stem cells by the piggyBac transposon." Nature Methods 6, no. 5 (2009): 363...
  7. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...from AAV2) Mammalian Transposon pRDA_757 (Piggybac; constitutive expression) Mammalian Plasmid...
  8. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ... CRISPR Competitor NgAgo Learn How You Can Use piggyBac Vectors in Genome Editing Resources on Addgene.org...
  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...lead to comparable experimental results. Using PiggyBac transposons, the Woltjen Lab compared different...reprogramming discrepancies. The polycistronic piggyBac transposon vectors tested in this work are available...
  10. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...uses the piggybac transposase to integrate dCas9-VPR into the genome of target cells. The piggybac system...McMahon A, et al. Cell. 2016. PubMed PMID: 27040499 PiggyBac mediated delivery of robust CRISPR activators ...
Showing: 1 - 12 of 12 results