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Plasmid#58867PurposeAAV expression of GFP under the Syn promoterDepositorInsertGFP
UseAAVTagsExpressionMammalianMutationPromoterSynAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFD1-dU6:1gRNA
Plasmid#49408Purposeexpression of gRNA under control of the Drosophila U6:1 promoterDepositorInsertdU6-1:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6-1Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAM1
Plasmid#60487PurposeThis plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genomeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgG2/κ
Plasmid#61884PurposeExpresses HER2/neu receptor specific humanized IgG2/κ antibody isotype (Trastuzumab-IgG2/κ)DepositorInsertsHER2/neu receptor specific humanized Gamma 2 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
UseTagsExpressionMammalianMutationPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF1983
Plasmid#143562PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTBX5 (TBX5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GFP-Sec61β
Plasmid#172442PurposeExpression of ER protein Sec61beta with an N-terminal FKBP-GFP tagDepositorInsertProtein transport protein Sec61 subunit beta
UseTagsFKBP-GFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKm-HCBE
Plasmid#170333Purposea modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium melilotiDepositorInsertRhizobium codon-optimized CBEs
UseCRISPRTagsExpressionMutationCas9n-D10APromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
IP3 receptor, type 1 scFv [L24/21]
Plasmid#206790PurposeMammalian Expression of IP3 receptor, type 1 scFV. Derived from hybridoma L24/21 scFv.DepositorInsertIP3 receptor, type 1 (Rattus norvegicus) recombinant scFV (Itpr1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-14-3-3 beta GST
Plasmid#13276DepositorInsert14-3-3 beta (YWHAB Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRBBm59
Plasmid#48115PurposeShuttle vector E. coli/B.meg.DepositorInsertpromoter sacB - gfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2 RFP670
Plasmid#187646Purposelentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning siteDepositorInsertRFP 670
UseLentiviralTagsRFP670 and sgRNA cloning siteExpressionMutationPromoterEFS (P2A)Available sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCH1/RAD51o
Plasmid#102562PurposeExpress Human RAD51 protein codon optimzed for E.coli and the E.coli GorE OperonDepositorInsertsHomo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
E. coli groE operon
UseTagsExpressionBacterialMutationCodon optimized for E.coli ExpressionPromoterT7Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOTTC414 - pAAV EF1a hPREP
Plasmid#59967PurposeAn AAV packaging vector that expresses hPREP under control of the EF1a promoter.DepositorInsertProlyl oligopeptidase (PREP Human)
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX303
Plasmid#25897DepositorTypeEmpty backboneUseLentiviral; Gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin
Plasmid#134868PurposeExpresses EGFP-tagged lysosomal markerDepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTOX10
Plasmid#127455PurposeEncodes YhaV toxin. Gent-RDepositorInsertyhaV toxin
UseTagsExpressionBacterialMutationPromoterpRhaAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.EF1a.ChR2-YFP.WPRE.hGH
Plasmid#100056PurposeAAV expression of ChR2 fused to YFP driven byEF1a promoter for optogenetic activationDepositorInsertChR2
UseAAVTagsEYFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 nickase for Split-PE (pJUL2497)
Plasmid#190104PurposeExpresses nickase SpCas9(H840A) for Split-PE useDepositorInsertnSpCas9
UseTagsXTEN-bpNLS and bpNLSExpressionMammalianMutationH840APromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
secretedBirA-8his
Plasmid#32408DepositorInsertBirA
UseTags8 His tagExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HDRT_TRAC_CD19-CAR
Plasmid#183473PurposeEncodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC geneDepositorUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001148862)
Plasmid#80263Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin
Plasmid#133027PurposeExpresses EGFP-tagged late endosome markerDepositorInsertRAB7A (RAB7A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-7aa-ActA-pEGFP-C1
Plasmid#177406PurposeTo express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4-5HT3 HC IRES EGFP
Plasmid#119740PurposeChemogenetics expression plasmidDepositorUseTagsExpressionMammalianMutationL131G, Q139L, Y217F, R420Q, R424D, R428APromoterCAGAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mNaChBac-T2A-tdTomato
Plasmid#60650PurposeExpresses mNaChBac-T2A-tdTomatoDepositorInsertmNaChBac-T2A-tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX552-GFP
Plasmid#107023PurposepX552-CMV-GFP. AAV plasmid for sgRNA cloning, with GFP fusion facilitating FACS sorting of cells. Replaced the hSyn promoter in pX552 from Zhang lab with a CMV promoter.DepositorInsertGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX - T7pro - EBFP
Plasmid#138615PurposeExpress EBFP with an upstream T7 promoter in a lentiviral backboneDepositorInsertEBFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMega-MaPylRS
Plasmid#200225PurposeThe plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.DepositorInsertsPyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA
UseTagsnoneExpressionBacterialMutationnonePromoterproK-lacO and tacIAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-4:TUBA1B-mEGFP
Plasmid#87421PurposeHomology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1BDepositorInsertTUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF2199
Plasmid#141967PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRXRA (RXRA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only