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Addgene

pLX303
(Plasmid #25897)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25897 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLKO
  • Backbone size (bp) 9335
  • Vector type
    Mammalian Expression, Lentiviral ; Gateway Destination vector
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdB resistant bacteria. 30C for liquid culture. 37C for plates.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a Stop codon directly after the Gateway sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX303 was a gift from David Root (Addgene plasmid # 25897 ; http://n2t.net/addgene:25897 ; RRID:Addgene_25897)
  • For your References section:

    A public genome-scale lentiviral expression library of human ORFs. Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE. Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638 10.1038/nmeth.1638 PubMed 21706014