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Differences in the mechanisms of proapoptotic BH3 proteins binding to Bcl-XL and Bcl-2 quantified in live MCF-7 cells.

Aranovich A, Liu Q, Collins T, Geng F, Dixit S, Leber B, Andrews DW
Mol Cell. 2012 Mar 30;45(6):754-63. doi: 10.1016/j.molcel.2012.01.030. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
177406mCherry-7aa-ActA-pEGFP-C1To express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.
177409Venus-BclXL-pEGFP-C1To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.
177417Venus-Bcl2-pEGFP-C1To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-2.

Antibodies from Article