pRBBm59
(Plasmid
#48115)
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PurposeShuttle vector E. coli/B.meg.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48115 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMM1520
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Backbone manufacturerMalten et al., 2005
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Growth instructionsFor Bacillus megaterium: Tetracycline and 37 °C
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepromoter sacB - gfp
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRBBm59 was a gift from Dieter Jahn (Addgene plasmid # 48115 ; http://n2t.net/addgene:48115 ; RRID:Addgene_48115) -
For your References section:
A sucrose-inducible promoter system for the intra- and extracellular protein production in Bacillus megaterium. Biedendieck R, Gamer M, Jaensch L, Meyer S, Rohde M, Deckwer WD, Jahn D. J Biotechnol. 2007 Dec 1;132(4):426-30. Epub 2007 Jul 10. 10.1016/j.jbiotec.2007.07.494 PubMed 17692983