pET29b_Qcat1
(Plasmid
#45129)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45129 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET29b
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Backbone manufacturerGenscript
- Backbone size w/o insert (bp) 5330
- Total vector size (bp) 6666
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BLR DE3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameE-coli-pathways-QconCAT1
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Alt nameQcat1
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SpeciesSynthetic
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Insert Size (bp)1336
- Promoter T7
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Tag
/ Fusion Protein
- 6XHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer GATCCCGCGAAATTAATACGACTCACTA
- 3′ sequencing primer GGTGGTGGTGGTGCTCGA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Synthesized by Genscript
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET29b_Qcat1 was a gift from Christopher Petzold (Addgene plasmid # 45129 ; http://n2t.net/addgene:45129 ; RRID:Addgene_45129) -
For your References section:
A targeted proteomics toolkit for High-throughput absolute quantification of Escherichia coli proteins. Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ. Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 10.1016/j.ymben.2014.08.004. 10.1016/j.ymben.2014.08.004 PubMed 25205128