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Plasmid#120234PurposeCarries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.DepositorInsertpromoter cassette containing ermE*p promoter and ampicillin resistance gene
UseSynthetic BiologyTagsExpressionMutationbla (amp resistance gene)PromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-C5E-6XTUBE
Plasmid#217628PurposeBacterial expression of MBP tagged 6XTUBEDepositorInsert6XTUBE
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70
Plasmid#62915PurposeCreating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2DepositorInsertT2A-p65AD-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
R26-H2B-EGFP HR donor vector
Plasmid#137925PurposeR0SA26 knock-in vector with homologous recombination-based methodDepositorInsertSA-H2B-EGFP-bpA with homology arms
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HDAC6.DC-FLAG
Plasmid#30483DepositorInsertHDAC6 (HDAC6 Human)
UseTagsFLAGExpressionMammalianMutationCatalytically inactive mutant (H216/611A)PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)
Plasmid#62956PurposeSinglet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP670-tubulin
Plasmid#136573PurposeHuman alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertemiRFP670-tubulin
UseTagsemiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSC-humanADAR1
Plasmid#198235PurposeExpresses human ADAR1 in yeastDepositorInserthuman ADAR1 (ADAR Human)
UseTagsHis10_TEVExpressionYeastMutationPromoterGAL1Available sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-Rac1-2G
Plasmid#66110PurposeSecond generation Rac1 FRET biosensorDepositorInsertSecond generation Rac1 FRET biosensor
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-fRCaMP2
Plasmid#125250PurposeExpresses red fluorescent calcium sensor in mammalian cellsDepositorInsertfRCaMP2
UseTagsExpressionMammalianMutationD396APromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-FOXO3a WT
Plasmid#1790DepositorInsertFOXO3a (FOXO3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2005AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1a-NICD1
Plasmid#46047DepositorInsertNotch1 (C-term) (Notch1 Mouse)
UseTagsMyc tagExpressionMammalianMutationCytoplasmic domain initiating at amino acid 1753PromoterEF1aAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG RUNX3 (R122C)
Plasmid#203438PurposeMammalian expression of RUNX3DepositorInsertRUNX3 (RUNX3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVP91A-pcaHG
Plasmid#113766PurposeExpresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.DepositorInsertsβ subunit of Protocatechuate 3,4- Dioxygenase
α subunit of Protocatechuate 3,4- Dioxygenase
UseTagsMet-6xHisExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CCR5
Plasmid#98943PurposeMammalian expression plasmid for untagged human CCR5DepositorInsertCCR5 (CCR5 Human)
UseTagsExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Flag-cMyc T58A
Plasmid#20076DepositorInsertcMyc (MYC Human)
UseRetroviralTagsFlagExpressionMammalianMutationT58APromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-FLNa WT
Plasmid#8982DepositorInsertFilamin A (FLNA Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRT498-OtUBD
Plasmid#190089PurposeExpresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX_TRE3G_DD::mCherry
Plasmid#108679PurposeTunable system for controlled gene expression and protein stability in mammalian cellsDepositorInsertDestabilizing Domain (DD) mCherry fusion protein
UseLentiviralTagsDD::mCherry fusion proteinExpressionMammalianMutationPromoterDoxycycline inducibleAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
scFLARE1
Plasmid#158738PurposeMammalian expression of scFLARE1 componentDepositorInsertscFLARE1
UseAAVTagsFlag, V5ExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only