pSC-humanADAR1
(Plasmid #198235)

• Purpose: Expresses human ADAR1 in yeast
• Depositing Lab: Brenda Bass
• Publication: Eggington et al Nat Commun. 2011;2:319. doi: 10.1038/ncomms1324.

Backbone

• Vector backbone: pBR322
• Backbone manufacturer: Herbert Boyer at the University of California, San Francisco
• Backbone size w/o insert (bp): 4361
• Modifications to backbone: plasmid is based on the YEpTOP2PGAL1 vector used to express DNA topoisomerase (Giaever GN, Snyder L, Wang JC. DNA supercoiling in vivo. Biophys Chem. 1988;29:7–15.)
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human ADAR1
• Alt name: ADAR
• Species: H. sapiens (human)
• Entrez Gene: ADAR (a.k.a. ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136)
• Promoter: GAL1
• Tag / Fusion Protein:
  • His10_TEV (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: TGGAAATGTAAAGAGCCCC
• 3′ sequencing primer: GAAGTGATCACCAAAGGC

Resource Information

• Supplemental Documents:
  • pSc-humanADAR1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry