pSc-humanADAR2
(Plasmid #171106)

• Purpose: Expresses human ADAR2 in yeast.
• Depositing Lab: Brenda Bass
• Publication: Macbeth et al Methods Enzymol. 2007;424:319-31. doi: 10.1016/S0076-6879(07)24015-7.

Backbone

• Vector backbone: pBR322
• Backbone manufacturer: Herbert Boyer at the University of California, San Francisco
• Backbone size w/o insert (bp): 4361
• Total vector size (bp): 11000
• Modifications to backbone: plasmid is based on the YEpTOP2PGAL1 vector used to express DNA topoisomerase (Giaever GN, Snyder L, Wang JC. DNA supercoiling in vivo. Biophys Chem. 1988;29:7–15.)
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human ADAR2
• Alt name: ADAR2
• Alt name: RED1
• Alt name: DRADA2
• Species: H. sapiens (human)
• Insert Size (bp): 2164
• GenBank ID: X99383.1
• Entrez Gene: ADARB1 (a.k.a. ADAR2, DRABA2, DRADA2, NEDHYMS, RED1)
• Promoter: GAL1
• Tag / Fusion Protein:
  • His12_TEV (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: TGGAAATGTAAAGAGCCCC
• 3′ sequencing primer: GAAGTGATCACCAAAGGC

Resource Information

• Supplemental Documents:
  • pSc-humanADAR2_12His_TEV.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry