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pEFIRES-P-ACSL3-mCherry

Plasmid

#87158

Purpose

Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ACSL3 (ACSL3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF-1-alpha

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO Myc ArA WT

Plasmid

#59804

Purpose

Allows the integration of Myc ArA in the genome and Tet-inducible expression

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora A (AURKA Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-synaptoPAC-minWPRE

Plasmid

#153100

Purpose

Cre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

Depositor

Dietmar Schmitz

Article

Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

Inserts

bPAC(WT)
Synaptophysin (Syp Rat)

Use

AAV

Tags

His tag, mScarlet, and myc tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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iOn-CAG∞RFP

Plasmid

#154014

Purpose

Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

mRFP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-myrGFP

Plasmid

#190233

Purpose

AAV vector, high-level expression of EGFP with a myristoylation/palmitoylation signal derived from Fyn N-terminus (myrGFP) in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

myristoylation signal sequence

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-HA.SV40(polyA)

Plasmid

#154850

Purpose

Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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LiOn-CAG∞GFP

Plasmid

#154015

Purpose

Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJR014

Plasmid

#198813

Purpose

Natural light-gated anion channels. In vivo inhibition of neurons using blue light.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::GtACR2::SL2::GFP::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-tetOn-IRES-G418 (EtO)

Plasmid

#84776

Purpose

Second generation lenti tetracycline operator (on)

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Insert

TET OPERATOR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

pTIGHT

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-PE5max-SF3B1-K700E

Plasmid

#200317

Purpose

Expresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.

Depositor

Lili Wang

Article

Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

Inserts

K700E-ng1+pg2
PEmax-2A-MLH1dn

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-ArchT-GFP

Plasmid

#29777

Purpose

AAV-mediated expression of ArchT-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV_Thy1StTA

Plasmid

#97411

Purpose

TET driver for amplified expression in cortical neurons

Depositor

Tetsuo Yamamori

Article

Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

Insert

tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

mouse thy1PSs

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP3-373

Plasmid

#91779

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

GENIE Project, Douglas Kim

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP3-373

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGV15 (CK2alpha K68M)

Plasmid

#27089

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha K68M (kinase dead) (CSNK2A1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K68M (kinase dead)

Promoter

CMV

Available since

May 17, 2012

Availability

Academic Institutions and Nonprofits only
pAAV2-SynTetOff-palGFP

Plasmid

#190232

Purpose

AAV vector, high-level expression of EGFP with a palmitoylation signal derived from GAP-43 N-terminus (palGFP) in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

palmitoylation signal sequence

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/T0-GFP-BRCA1

Plasmid

#71116

Purpose

mammalian expression of GFP-BRCA1 siRNA resistant construct

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-Flag-RNF168

Plasmid

#133976

Purpose

mammalian expression vector of Flag tagged wild type RNF168

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pWZ403

Plasmid

#208113

Purpose

mSwAP-In marker cassette 1 with mScarlet-PuroTK

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mScarlet-PuroTK

Use

Tags

P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-oScarlet

Plasmid

#137135

Purpose

Viral expression of oScarlet

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

oScarlet

Use

AAV

Tags

Expression

Mutation

E95D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-TRPV1-P2A-DsRed

Plasmid

#200830

Purpose

Transduce all neuron types with AAV to express TRPV1 along with a DsRed fluorescence reporter

Depositor

Hong Chen

Article

Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

Insert

TRPV1-P2A-DsRed (Trpv1 Rat)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Syn

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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