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pCMV3flag8HOIP

Plasmid

#50015

Purpose

Mammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

ring finger protein 31 (RNF31 Human)

Use

Tags

3 x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9

Plasmid

#75381

Purpose

dCas9

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

Sp dCas9

Use

Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-FOXM1

Plasmid

#153136

Purpose

Expression of Flag tagged human FOXM1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXM1 (FOXM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pPEgRNA

Plasmid

#172716

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-F-del Casp9.IRES.GFP

Plasmid

#15567

Depositor

David Spencer

Article

Straathof et al Blood. 2005 Jun 1. 105(11):4247-54.

Insert

FKBP12(V36)-p30Caspase9 (FKBP1A Human)

Use

Retroviral

Tags

FKBP12 and HA epitope

Expression

Mammalian

Mutation

FKBP12 F36 to V Truncated Caspase9 (135-416; lac…

Promoter

Available since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-KRAB-dCas9-P2A-mCherry

Plasmid

#60954

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Insert

KRAB-dCas9-P2A-mCherry fusion

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, KRAB domain, and mCherry

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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Cherry-Fibrillarin-C1

Plasmid

#134539

Purpose

Expresses Cherry-Fibrillarin fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Fibrillarin (FBL Human)

Use

Tags

Cherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12D

Plasmid

#81651

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G12D

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3G-LSD1-dCas9-P2A-mCherry

Plasmid

#138462

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherry

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

LSD1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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Z-lock cofilin

Plasmid

#141137

Purpose

controlling the actin-severing ability of cofilin with light

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Use

Tags

Expression

Mammalian

Mutation

mCherry, Zdk2(132F), Cofilin S3A, LOV WT

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Rabbit GFP Cav 2.2 pMT2

Plasmid

#58737

Purpose

expression of GFP tagged rabbit Cav2.2 in mammalian cells

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

Cav2.2 (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6b-V106W

Plasmid

#215825

Purpose

Express CBE6b V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-Pa1

Plasmid

#138475

Purpose

Enables high-efficiency recombineering in P. aeruginosa

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

PapRecT
mutL E36K

Use

Tags

Expression

Bacterial

Mutation

E36K

Promoter

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV_IRES_GFP_CD16a

Plasmid

#196189

Purpose

Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

Depositor

Ana Ruiz-Saenz

Article

Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

Insert

CD16a (FCGR3A Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Phenylalanine 158 to Valine

Promoter

LTR

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4

Plasmid

#27002

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4 (NEDD4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pMG36E

Plasmid

#133853

Purpose

Expression vector

Depositor

Gerard Venema, Jan Kok

Article

van de Guchte et al Appl Environ Microbiol. 1989 Jan;55(1):2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

P32

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.LifeAct-GFP.W

Plasmid

#51010

Purpose

Lentivector that expresses LifeAct-GFP from internal human PGK promoter

Depositor

Rusty Lansford

Insert

LifeAct-GFP

Use

Lentiviral

Tags

LifeAct

Expression

Mammalian

Mutation

Promoter

human PGK

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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Sfp pet29b C-terminal His Tag

Plasmid

#75015

Purpose

Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

Depositor

Michael Burkart

Article

Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

Insert

Sfp

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-coilin

Plasmid

#36906

Depositor

Greg Matera

Article

Shpargel et al J Cell Sci. 2003 Jan 15;116(Pt 2):303-12

Insert

coilin (COIL Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

Plasmid

#138460

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-KRAB-IRES-zsGreen1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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