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Showing: 21 - 40 of 157954 results

  1. Tet-pLKO-puro

    Plasmid
    #21915
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Depositor
    Dmitri Wiederschain
    Article
    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    21915-plasmid-map-sequence-id-326721

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  3. pUMVC

    Plasmid
    #8449
    Purpose
    Packaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2005
    Availability
    Academic Institutions and Nonprofits only
    8449-plasmid-map-sequence-id-272509

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  5. lentiCRISPR v2

    Plasmid
    #52961
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52961-plasmid-map-sequence-id-244694

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  7. LentiGuide_DKO

    Plasmid
    #183193
    Purpose
    Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Article
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6, mU6
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183193-plasmid-map-sequence-id-360099

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  9. pTUM4

    Plasmid
    #230905
    Purpose
    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli
    Depositor
    Arne Skerra
    Article
    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-
    Inserts
    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    230905-plasmid-map-sequence-id-460506

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  10. pX330-U6-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #42230
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.
    Depositor
    Feng Zhang
    Article
    Cong et al Science. 2013 Jan 3.
    Has Service
    Cloning Grade DNA
    Insert
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    42230-plasmid-map-sequence-id-419908

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  16. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Article
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

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  17. pCMV-15F11-HA-mEGFP

    Plasmid
    #129590
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129590-plasmid-map-sequence-id-254270

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  18. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Depositor
    Eric Lander, David Sabatini
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71409-plasmid-map-sequence-id-322475

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Showing: 21 - 40 of 157954 results