Showing: 5901 - 5920 of 13935 results
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Plasmid#113718PurposeBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationD10A, N580APromoterAvailabilityAcademic Institutions and Nonprofits only -
pY003 (pFnCpf1_delta Cas)
Plasmid#69974PurposeExpresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.DepositorInsertFnCpf1 locus without Cas genes
UseCRISPRTagsExpressionBacterialMutationCas genes are deleted from locusPromoterAvailabilityAcademic Institutions and Nonprofits only -
pY002 (pFnCpf1_min)
Plasmid#69975PurposeExpresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.DepositorInsertFnCpf1 locus without Cas genes nd artificial promoter
UseCRISPRTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGreenIIM DR5v2-ntdTomato/DR5-n3GFP
Plasmid#61628PurposeExpresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoterDepositorInsertsntdTomato
n2xEGFP
UseTagsExpressionPlantMutation2 copies of EGFP with N-terminal SV40 NLSPromoterAuxin-dependent DR5 promoter containing 9 reverse…AvailabilityAcademic Institutions and Nonprofits only -
Fv-hCaspase 8-2A-GFP
Plasmid#82712Purposeexpress human caspase 8 in mammalian cellsDepositorInsertFv-Caspase 8-2A-GFP (CASP8 Human)
UseRetroviralTagsFKBP dimerization domainExpressionMammalianMutationdeleted amino acids 1-215 of caspase 8PromoterLTRAvailabilityAcademic Institutions and Nonprofits only -
Fv-hCaspase 8 (C/A)-2A-GFP
Plasmid#82713Purposeexpress human caspase 8 in mammalian cellsDepositorInsertFv-Caspase 8 (C/A)-2A-GFP (CASP8 Human)
UseRetroviralTagsFKBP dimerization domainExpressionMammalianMutationdeleted amino acids 1-215 of caspase 8PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV-hARdeltaNH2-6
Plasmid#89104Purposemammalian expression of human androgen receptor amino acids 507-919DepositorInserthAR DNA binding domain+ligand binding domain (AR Human)
UseTagsExpressionMammalianMutationNH2 terminal deletion, contains amino acids 507-…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
VN-alpha Synuclein
Plasmid#89470Purposeexpresses human alpha Synuclein fused to the N-terminal part of VenusDepositorInsertSNCA (SNCA Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
alpha Synuclein-VC
Plasmid#89471Purposeexpresses human alpha Synuclein fused to the C-terminal part of VenusDepositorInsertSNCA (SNCA Human)
UseTagsC-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pICSL11055
Plasmid#68252PurposeLevel 1 Golden Gate Cassette: Plant kanamycin resistance cassetteDepositorInsertPromoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pICSL11056
Plasmid#68258PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plantsDepositorInsertPromoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-YOD1-C160S
Plasmid#85666PurposeExpression of human YOD1 C160S mutant with N-terminal GFP tagDepositorInsertYOD1 (YOD1 Human)
UseTagsGFPExpressionMammalianMutationC160SPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-YOD1-MutS2
Plasmid#85667PurposeExpression of human YOD1 I292Q V295Q mutant with N-terminal GFP tagDepositorInsertYOD1 (YOD1 Human)
UseTagsGFPExpressionMammalianMutationI292Q and V295QPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCS2+ oDam_f_GFPoNLS
Plasmid#85819PurposeiDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertoDam_f_GFPoNLS
UseTagsoNLS (optimized nuclear localization signal) C te…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc Dam_f_GFPoNLS
Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A.PromoterAvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(D3A: D175A D28A D9A)
Plasmid#90090PurposeExpresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationD175A D28A D9APromoterAvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-ADGRL3-Flag
Plasmid#201218PurposepCDNA3.1 with kozak (GCC), ADGRL3 and C-terminal FlagDepositorInsertADGRL3 (Adgrl3 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
ADGRL3-CTF-Flag
Plasmid#201219PurposepCDNA3.1 with kozak (GCC), ADGRL3 C-terminal fragment and C-terminal FlagDepositorInsertADGRL3 (Adgrl3 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
SpIMPDH
Plasmid#206980PurposeStreptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.DepositorInsertSpIMPDH (guaB Streptococcus pyogenes)
UseTagsHis tag (with TEV cleavage site)ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
B-HiFi SpCas9
Plasmid#207362PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HiFi SpCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationR691A, amino acids 1005-1013 replaced with two gl…PromoterCBhAvailabilityAcademic Institutions and Nonprofits only
Showing: 5901 - 5920 of 13935 results