Addgene

Showing: 5901 - 5920 of 14536 results

Results per page:

Sort results by:

pBJG1 T7-8xHis-OGT K842M

Plasmid

#154272

Purpose

Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

Depositor

Suzanne Walker

Article

Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi

Insert

O-GlcNAc Transferase (OGT Human)

Use

Tags

8x His, HRV3C protease site, and T7 leader

Expression

Bacterial

Mutation

Lysine 842 changed to Methionine- bacterial codon…

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PD-1-miSFIT-1x

Plasmid

#124679

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry

Plasmid

#176277

Purpose

Viral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

MCS-P2A-mCherry

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

human Synapsin I

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-1_VNp-hGH

Plasmid

#182411

Purpose

Bacterial expression of Vesicle Nucleating peptide-human growth hormone fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-hGH (GH1 Human)

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_eA3A T31A-dCas9

Plasmid

#163537

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A T31A-dCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

dCas9 (D10A, H840A)eA3A T31A

Promoter

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Delta(Pro-MP)ADAM10-HA

Plasmid

#65107

Purpose

Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM10 (ADAM10 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 19-455

Promoter

CMV

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only
AAV2-miniSOG-VAMP2-T2A-mCherry

Plasmid

#50970

Purpose

AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-T2A-mCherry) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Insert

miniSOG-VAMP2-T2A-mCherry (Vamp2 Arabdopsis thaliana, Mouse)

Use

AAV

Tags

T2A-mCherry and miniSOG

Expression

Mutation

Promoter

human synapsin promoter

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EFS-DIO-GRAB_OTmut

Plasmid

#185389

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

AAV

Tags

Expression

Mutation

Promoter

EFS

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension

Plasmid

#176542

Purpose

Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Inserts

MBP tag
TnpB

Use

Tags

3' extension derived from endogenous locus a…

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

Plasmid

#146392

Purpose

Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

Insert

DmeIF4G-trunc (eIF4G1 Fly)

Use

Tags

Expression

Insect

Mutation

322-1666 truncated version of elF4G sequence with…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-Sniper SpCas9-NLS-6xHis

Plasmid

#207374

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

Plasmid

#182925

Purpose

Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Type

Empty backbone

Use

Tags

APEX2 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PITX3-eGFP-PGK-Puro

Plasmid

#31942

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Insert

PITX3 (PITX3 Human)

Use

Targeting donor

Tags

eGFP

Expression

Mutation

Promoter

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-eSpCas9-plus-NLS-6xHis

Plasmid

#207380

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9-plus

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, R1060A, amino acids 1005-1013 replaced wit…

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR366 RNF2

Plasmid

#47509

Purpose

human gRNA expression vector targeting RNF2

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-RNF2 (RNF2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Basic-hCYP26A1-FL- luciferase

Plasmid

#135566

Purpose

Full Length promoter of the human CYP26A1 gene in pGL3-Basic-luciferase

Depositor

Catharine Ross

Article

Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51

Insert

Full-length promoter of human retinoic acid receptor-beta gene

Use

Tags

Expression

Mutation

Promoter

hHuman CYP26A1 gene promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-CD81-(TEV)-FKBP-Myc

Plasmid

#177908

Purpose

human CD81-TEV cleavage site-FKBP-Myc tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

human CD81 (CD81 Synthetic, Human)

Use

Tags

FKBP, Myc tag, and TEV protease cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCVL.SSA TLR (Sce)

Plasmid

#45576

Purpose

Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

5' iRFP arm
eGFP with I-Sce I TS
+3 mCherry
3' iRFP arm

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

embedded I-Sce I TS from 163-185bp, truncated 25 …

Promoter

SFFV

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetOminiCMV-OS

Plasmid

#136576

Purpose

Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX_317-C7orf26v1

Plasmid

#178537

Purpose

Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_317 backbone

Depositor

William Hahn

Article

Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

Insert

C7orf26v1 (INTS15 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge