pRSFDuet-1_VNp-hGH
(Plasmid
#182411)
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PurposeBacterial expression of Vesicle Nucleating peptide-human growth hormone fusion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 182411 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSFDUET-1
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVNp-hGH
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SpeciesH. sapiens (human)
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GenBank IDUniProtKB: P01241
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Entrez GeneGH1 (a.k.a. GH, GH-N, GHB5, GHN, IGHD1A, IGHD1B, IGHD2, hGH-N)
- Promoter T7
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Tag
/ Fusion Protein
- Vesicle Nucleating peptide (VNp) (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSFDuet-1_VNp-hGH was a gift from Dan Mulvihill (Addgene plasmid # 182411 ; http://n2t.net/addgene:182411 ; RRID:Addgene_182411) -
For your References section:
High-yield vesicle-packaged recombinant protein production from E. coli. Eastwood TA, Baker K, Streather BR, Allen N, Wang L, Botchway SW, Brown IR, Hiscock JR, Lennon C, Mulvihill DP. Cell Rep Methods. 2023 Feb 2;3(2):100396. doi: 10.1016/j.crmeth.2023.100396. eCollection 2023 Feb 27. 10.1016/j.crmeth.2023.100396 PubMed 36936078