-
Plasmid#185388PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neuronsDepositorHas ServiceAAV1 and AAV9InsertGPCR activation based oxytocin control sensor GRAB_OTmut
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pColdI-GST-mGTSF1
Plasmid#214671PurposeBacterial expression of GST-tagged mouse GTSF1DepositorInsertGTSF1 (Gtsf1 Mouse)
UseTags6x His and GSTExpressionBacterialMutationPromoterCspAAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSynapsin_NLS_GFP_P2A_SNAP_NR1
Plasmid#170458PurposeExpresses SNAP tagged NR1 in neuronal cellsDepositorInsertNR1 (Grin1 Rat)
UseLentiviralTagsSNAPExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DsRed_IRES_MAPT:EGFP
Plasmid#92196PurposeLentiviral overexpression vector to make stable bicistronic cell line for Tau screenDepositorInsertDsRed-IRES-MAPT-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-Anc689-UdgX-nCas9-RBMX
Plasmid#163552PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-Anc689-UdgX-nCas9-RBMX
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-HF1
Plasmid#138556PurposeExpresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-HF1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926APromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3
Plasmid#127356Purposegateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and mVenusExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-Flag-Cdk9-IRES-eGFP
Plasmid#28096DepositorInsertCdk9 (CDK9 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pICSL11056
Plasmid#68258PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plantsDepositorInsertPromoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cre-p2a-tdTomato
Plasmid#176837PurposeExpress Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expressionDepositorInsertCre-P2A-tdTomato
UseTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW V5 Eco/Dam mse EMD
Plasmid#182675PurposeLentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.DepositorInsertEmerin (Emd Mouse)
UseLentiviralTagsE. Coli Dam and V5ExpressionMammalianMutationPromoterHSPAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
PUC57_BAG3wt-eGFP
Plasmid#98182PurposeCodes for full length, GFP tagged, human wildtype Bag3DepositorInsertBAG3wt-eGFP (BAG3 Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-GalT-IRES-Puromycin
Plasmid#134862PurposeExpresses EGFP-tagged Golgi markerDepositorInsertbeta 1,4-galactosyltransferase (B4GALT1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 83-398PromoterEF1aAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-tagRFP-BleoR
Plasmid#192508PurposeExpression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.DepositorInsertPE2-P2A-tagRFP-P2A-BleoR
UseTagsBleoR, SV40 bpNLS, and tagRFPExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-actin
Plasmid#118378PurposeGateway compatible donor vector with WT actin.DepositorInsertactin beta (ACTB Human)
UseDonor-plasmidTagsExpressionMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-SsAPOBEC3B
Plasmid#198550PurposeExpresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-SsAPOBEC3B
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSI-Target-AID-NG
Plasmid#119861PurposeExpresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.DepositorInsertnSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAPDH::H2B-mScarlet
Plasmid#186764PurposeA plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.DepositorInsertH2B-mScarlet
UseOverexpressionTagsH2BExpressionMutationPromoterGAPDHAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD81-(TEV)-FKBP-SmBiT
Plasmid#182651Purposehuman CD81-TEV cleavage site-FKBP-SmBiT tagDepositorInserthuman CD81-TEVp cleavage site-FKBP-SmBiT (CD81 Synthetic, Human)
UseTagsSmBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST PTBP1 isoform 4
Plasmid#108594PurposeRecombinant GST tagged protein productionDepositorInsertPTBP1 isoform 4 (PTBP1 Human)
UseTagsGSTExpressionBacterialMutationisoform 4 (isoform a)PromotertacAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only