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Engineered CRISPR-Cas9 nuclease with expanded targeting space.

Nishimasu H, Shi X, Ishiguro S, Gao L, Hirano S, Okazaki S, Noda T, Abudayyeh OO, Gootenberg JS, Mori H, Oura S, Holmes B, Tanaka M, Seki M, Hirano H, Aburatani H, Ishitani R, Ikawa M, Yachie N, Zhang F, Nureki O
Science. 2018 Aug 30. pii: science.aas9129. doi: 10.1126/science.aas9129. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
117919pX330-SpCas9-NGExpresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.
119861pSI-Target-AID-NGExpresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

Antibodies from Article