Showing: 561 - 580 of 13875 results
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Plasmid#171585PurposeExpression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporterDepositorInsertTRS-Leader-SARS-CoV-2-d2eGFP
UseLentiviralTags2PEST AND NLSExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)
Plasmid#187844PurposeExpression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
UseTagsIg k-chain leader sequence, SNAPf-tag, and meGFPe…ExpressionMammalianMutationmeGFPe: asparagine 198 to aspartic acid and tyros…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Muc5b D1 (murine) with cleavable His tag
Plasmid#181814PurposeExpresses murine Muc5b D1 with cleavable His tag, residues 50-426DepositorInsertMuc5b D1 (Muc5b Mouse)
UseTagsTEV cleavage site followed by 6xHistidine tagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1D2D' with cleavable His tag
Plasmid#187993PurposeExpresses MUC2 D1D2D' assembly with cleavable His tag in mammalian cellsDepositorInsertMucin2 D1D2D' (MUC2 Human)
UseTags6xHistadine followed by TEV cleavage siteExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag
Plasmid#187994PurposeExpresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cellsDepositorInsertmucin2 D1D2D'D3 (MUC2 Human)
UseTags6xHistadine followed by TEV cleavage siteExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)
Plasmid#192704PurposeExpression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
UseTagsIg k-chain leader sequence, SNAPf-tag, and meGFPm…ExpressionMammalianMutationmeGFPm: gluatmic acid 142 to asparagine and histi…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
α-actinin-4-M522-sstFRET(Venus/Cerulean) in pUNO1
Plasmid#190799PurposeImaging of force-sensitive α-actinin-4 in live cellsDepositorInsertα-actinin-4-Venus-Cerulean-522 (ACTN4 Synthetic, Human)
UseTagscerulean and venusExpressionMammalianMutationPromoterhEF1-HTLVAvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)
Plasmid#192784PurposeExpression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
UseTagsIg k-chain leader sequence, SNAPf-tag, and mXFPmExpressionMammalianMutationmXFPm: tryptophan 66 to phenylalanine, gluatmic a…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)
Plasmid#192785PurposeExpression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
UseTagsIg k-chain leader sequence, SNAPf-tag, and mXFPeExpressionMammalianMutationmXFPe: tyrosine 66 to phenylalanine, asparagine 1…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-mEGFP-MBP-TMD(ALA7)
Plasmid#193051PurposeExpression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.DepositorInsertALA7-TMD
UseTagsIg k-chain leader sequence, MBP, and mEGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1
Plasmid#79379PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertChimera 1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2
Plasmid#79380PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertChimera 2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4
Plasmid#79382PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertChimera 4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full
Plasmid#79383PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertZhang 2.0 + scaffold
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSrpR-BCD20-Cerulean-ssrA P65U23C89T19 v50-2a (587)
Plasmid#70744Purposefor building 3-color reporter plasmidDepositorInsertCerulean-ssrA
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG
Plasmid#127326PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader GUG-nLuc-3XFLAG
Plasmid#127327PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)
Plasmid#126025Purposeexpress RFP- and mCer-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-mCerulean-MP; CRY2 PHR-VHH(GFP)
UseTagsRFPExpressionInsectMutationWTPromoterHsp70AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124428PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry
Plasmid#124429PurposeExpresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(TEV cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 561 - 580 of 13875 results