Four-color single-molecule imaging with engineered tags resolves the molecular architecture of signaling complexes in the plasma membrane.
Sotolongo Bellon J, Birkholz O, Richter CP, Eull F, Kenneweg H, Wilmes S, Rothbauer U, You C, Walter MR, Kurre R, Piehler J
Cell Rep Methods. 2022 Feb 4;2(2):100165. doi: 10.1016/j.crmeth.2022.100165. eCollection 2022 Feb 28. PubMed Article
Sotolongo Bellon J, Birkholz O, Richter CP, Eull F, Kenneweg H, Wilmes S, Rothbauer U, You C, Walter MR, Kurre R, Piehler J
Cell Rep Methods. 2022 Feb 4;2(2):100165. doi: 10.1016/j.crmeth.2022.100165. eCollection 2022 Feb 28. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187844 | pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557) | Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy |
| 192704 | pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557) | Expression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy |
| 192784 | pSems-leader-SNAPf-mXFPm-IFNAR1(28-557) | Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy |
| 192785 | pSems-leader-SNAPf-mXFPe-IFNAR1(28-557) | Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy |
| 192786 | pSems-leader-mXFPm-IFNGR1(18-489) | Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy |
| 192787 | pSems-leader-mXFPe-IFNGR2(30-337) | Expression of mXFPe-tagged IFNGR2 at the plasma membrane, e.g. for single molecule fluorescence microscopy |
| 192788 | pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6 | aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling |
| 192789 | pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6 | aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling |
| 193051 | pSems-leader-mEGFP-MBP-TMD(ALA7) | Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy. |