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Plasmid#24603DepositorUseCre/Lox and LentiviralTagsExpressionMammalianMutationOct4-2A-KLF4-2A-Sox2-IRES-MycPromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only
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pαH-S-GSAS-B.1.1.7
Plasmid#171747PurposeMammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)DepositorInsertSpike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/XBB.1.16-RBD
Plasmid#213070PurposeMammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variantDepositorInsertpCAGGS/XBB.1.16-RBD (S )
UseTags6 His TagExpressionMammalianMutationG339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.351.v2
Plasmid#173786PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 2DepositorInsertSpike (S-GSAS-B.1.351 variant version 2) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.351
Plasmid#171749PurposeMammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-B.1.351 variant (South African)DepositorInsertSpike (S-GSAS-B.1.351 variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-D614G-K417N-E484K-N501Y
Plasmid#171750PurposeMammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-K417N-E484K-N501YDepositorInsertSpike (S-GSAS-D614G-K417N-E484K-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-D614G-deltaFV
Plasmid#171743PurposeMammalian expression of SARS-CoV-2 Spike protein mink variant (S-GSAS-D614G-deltaFV variant)DepositorInsertSpike (S-GSAS-D614G-deltaFV variant) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/hFGFR3-K650M-V5
Plasmid#214909Purposeexpression of the K650M mutant variant of human FGFR3, which is associated with Severe Achondroplasia with Developmental Delay and Acanthosis Nigricans (SADDAN)DepositorInsertFGFR3 (FGFR3 Human)
UseTagsV5/HisExpressionMammalianMutationK650M substitutionPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-2P-Omicron-BA.5
Plasmid#213828PurposeMammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variantDepositorInsertpαH-S-GSAS/Omicron-2P-BA5 (S )
UseTagsHRV 3C cleavage site (before tags) 8X His tag 2X…ExpressionMammalianMutationG339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-rS2d
Plasmid#183516PurposeMammalian cell expression of SARS-CoV-2 Spike protein S-GSAS-rS2d with (682-685) furin side replaced with GSAS and mutation S383C, D985CDepositorInsertrS2d (S Severe acute respiratory syndrome coronavirus 2)
UseTags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
GEARBOCS-SPARCL1-GeneTRAP
Plasmid#218184PurposeTo KO and genetrap mouse Hevin simultaneouslyDepositorInsertsgRNA (Sparcl1 Mouse)
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAC212
Plasmid#187912Purposepsac212-crispey-bar-ade2KODepositorInsertade2 KO CRISPEY cassette
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Gfap-GeneTRAP
Plasmid#196492PurposeTo KO and genetrap mouse GFAP simultaneouslyDepositorInsertsmCherry-CAAX
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Vamp2-GeneTRAP
Plasmid#196493PurposeTo KO and genetrap mouse VAMP2 simultaneouslyDepositorInsertsmCherry-CAAX
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-OMICRON.BA.5
Plasmid#213069PurposeMammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 variantDepositorInsertS-GSAS-Omicron.BA5 (S )
UseTagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR/XBB.1.16
Plasmid#213037PurposeMammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.16 with with furin side intactDepositorInserts -RRAR.XBB.1.16 (S )
UseTagsHRV 3C cleavage site (before tags) 8X His tag 2X…ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 furin site RRAR …PromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR-OMICRON.BA.5
Plasmid#213071PurposeMammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 with with furin side intactDepositorInsertpαH-S-RRAR-OMICRON.BA.5 (S )
UseTagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685, furin site inta…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-OMICRON.BA.2-RBD
Plasmid#184830PurposeMammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorInsertS-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
UseTags6X His tagExpressionMammalianMutationSARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…PromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-OMICRON.BA.1-RBD
Plasmid#184831PurposeMammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorInsertS-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
UseTags6X His tagExpressionMammalianMutationSARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…PromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pαH-rS2d-HexaPro
Plasmid#183515PurposeMammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987PDepositorInsertSpike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
UseTags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only