....   The ICE tool also provides a Knockout score (KO-score) which represents the proportion of cells that...contributing indels are likely to result in a functional KO of the targeted gene.   Analyze Complex Edits   Traditional..., the more confident you can be in the ICE score. KO Score - Represents the proportion of cells that have...indels are likely to result in a functional Knockout (KO) of the targeted gene. The analysis can be sorted...

...vector (pRDA_526) that (1) enables efficient knockout (KO) of genes in immune cells; (2) is not immunogenic... vivo studies; (3) is compatible with single gene KO and pooled screening approaches; (4) encodes a Thy1.1...

...and SYT1 KO cells were labelled with a green or violet, fluorescent dye, respectively. WT and KO cells were...CRISPR-Cas9 approaches are commonly used to produce KO cell lines, some of which are commercially available...ensure that the parental cell lines of your chosen KO line are likely to express the target at a robust...representative images showing the staining intensity in the KO population (pink histogram) compared to the WT cells...that shows separation between the wild-type and the KO population (Figure 1), further optimization can be...

...other], Cas12a, RNA-targeting) CRISPR mechanism (KO, CRISPRa, CRISPRi, editing)   Figure 2: Selecting...

...different categories based on function: knockout (KO), activate, inhibit, base edit, and other. The first...

... Lab Human ubiquitination-related proteins CRISPR KO library 174592 Knockout Human Bonifacino 3rd 6-25...Mouse Adamson 3rd 1-20 per edit Varies CRASP-Seq Gene KO Library 232069 Knockout Human Gonatopoulos-Pournatzis...

...codon Entry vector Ko 139546 pENTR-CreStopDeleted Cre without stop codon Entry vector Ko 139547 pENTR-CreERT2...pENTR-CreERT2 Cre-ERT2 with stop codon Entry vector Ko 139670 pTFUbiCre Cre Maize Ubiquitin Plant Birchler 140131...

...Young-Gie Chung, Mi-Yoon Chang, Baek-Soo Han, Sanghyeok Ko, Eungi Yang, Kwang Yul Cha, Robert Lanza, and Kwang-Soo...

...16648 DNMT1 KO Hyg DNMT1 T7 Hereditary sensory neuropathy type IE Bert Vogelstein 16649 DNMT1 KO Neo DNMT1...TARDBP-Exon1-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9...