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pcDNA3.1-Flag-gp130dimer
Plasmid#174394PurposeCytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).DepositorInsertIL6ST cytosolic domain with leucine zipper & C-C linker
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Lmajor_gp63_10_0460_WT
Plasmid#171640PurposeExpression of wildtype L. major glcyoprotein-63 from chromosome 10 in mammalian cellsDepositorInsertLmjF.10.0460
UseTagsMyc-HisExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Lmajor_gp63_10_0460_E265A
Plasmid#171641PurposeExpression of catalytically inactive L. major glcyoprotein-63 (E265A) from chromosome 10 in mammalian cellsDepositorInsertLmjF.10.0460 E265A
UseTagsMyc-HisExpressionMammalianMutationE265APromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pDEST-PB-R4R3
Plasmid#139518PurposeProvides two PiggyBac transposon sequences between gene cassettes in the destination vectorDepositorTypeEmpty backboneUsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pWSK129-stm2585
Plasmid#174401PurposeS. Typhimurium stm2585 (sarA/steE) flanked with endogenous regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial expression.DepositorInsertsarA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pENTR-R2L3-pA-floxPGKneopA
Plasmid#139543PurposeProvides pA sequences N-terminal to floxed PGK-neo-pA cassette on Gateway entry vectorDepositorInsertNeomycin resistant gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TET-PH-lox66FRT
Plasmid#139561PurposepiggyBac expression vector with TET-ON system and puromycin selectionDepositorInsertPGK-puroR-pA cassette, inserted in PB-TET vector
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -