Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 6 results for: KO

Showing: 1 - 6 of 6 results

  1. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ....   The ICE tool also provides a Knockout score (KO-score) which represents the proportion of cells that...contributing indels are likely to result in a functional KO of the targeted gene.   Analyze Complex Edits   Traditional..., the more confident you can be in the ICE score. KO Score - Represents the proportion of cells that have...indels are likely to result in a functional Knockout (KO) of the targeted gene. The analysis can be sorted...

  2. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...vector (pRDA_526) that (1) enables efficient knockout (KO) of genes in immune cells; (2) is not immunogenic... vivo studies; (3) is compatible with single gene KO and pooled screening approaches; (4) encodes a Thy1.1...

  3. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...and SYT1 KO cells were labelled with a green or violet, fluorescent dye, respectively. WT and KO cells were...CRISPR-Cas9 approaches are commonly used to produce KO cell lines, some of which are commercially available...ensure that the parental cell lines of your chosen KO line are likely to express the target at a robust...representative images showing the staining intensity in the KO population (pink histogram) compared to the WT cells...that shows separation between the wild-type and the KO population (Figure 1), further optimization can be...

  4. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...other], Cas12a, RNA-targeting) CRISPR mechanism (KO, CRISPRa, CRISPRi, editing)   Figure 2: Selecting...

  5. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...different categories based on function: knockout (KO), activate, inhibit, base edit, and other. The first...

  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...Young-Gie Chung, Mi-Yoon Chang, Baek-Soo Han, Sanghyeok Ko, Eungi Yang, Kwang Yul Cha, Robert Lanza, and Kwang-Soo...
Showing: 1 - 6 of 6 results