pcDNA3.1/hFGFR3-K650M-V5
(Plasmid
#214909)
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Purposeexpression of the K650M mutant variant of human FGFR3, which is associated with Severe Achondroplasia with Developmental Delay and Acanthosis Nigricans (SADDAN)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214909 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1-V5-HIS-TOPO
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFGFR3
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SpeciesH. sapiens (human)
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MutationK650M substitution
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Entrez GeneFGFR3 (a.k.a. ACH, CD333, CEK2, HSFGFR3EX, JTK4)
- Promoter CMV
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Tag
/ Fusion Protein
- V5/His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1/hFGFR3-K650M-V5 was a gift from Pavel Krejčí (Addgene plasmid # 214909 ; http://n2t.net/addgene:214909 ; RRID:Addgene_214909)