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Plasmid#169124Purposeexpression of proteinDepositorInserthRad23A (RAD23A Human)
UseTagsExpressionBacterialMutationwtPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-tTA
Plasmid#99118PurposeAn AAV genome that ubiquitously expresses tTA from the CAG promoterDepositorHas ServiceAAV2InserttTA
UseAAVTagsExpressionMammalianMutation*(see below)PromoterCAGAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAS1yl
Plasmid#73226PurposeConstitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cellsDepositorInsertsCas9
sgRNA
UseCRISPR; In y. lipolyticaTagsExpressionBacterialMutationPromoterunknownAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PKCdelta-EGFP
Plasmid#178856PurposeMammalian expression of PKCδ fused to EGFPDepositorInsertPKCδ (PRKCD Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW-IRF4-Puro
Plasmid#123323PurposeLentiviral vector for expression of IRF4 under a Doxycycline inducible promoterDepositorInsertIRF4 (IRF4 Human)
UseLentiviralTagsExpressionMammalianMutationseveral silent mutations to disrupt sgRNA targeti…PromoterDoxycycline inducible minimal CMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-mCherry
Plasmid#109421PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-CSNK1A1 WT-Puro
Plasmid#123319PurposeLentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoterDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b-mLin28A-cHIS
Plasmid#137998PurposeLin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cellsDepositorInsertmouse Lin28A, CSD and ZnFs (residues 36–186)
UseTagsC-terminal His6 tagExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
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GFP-PKI nls
Plasmid#118301PurposeExpression of PKIaDepositorInsertPKI nls (aa 2-36)
UseTagsFlag and GFPExpressionMammalianMutationinserting 2xnlsPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-C-IFT88
Plasmid#218733PurposeExpresses N-terminally EGFP-tagged IFT88 in mammalian cellsDepositorInsertIFT88 (IFT88 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S in pAR9 (rep/cap)
Plasmid#174539Purposeencodes AAV-S capsid for efficient gene delivery to the mammalian cochleaDepositorInsertVP1 of AAV9 containing the AAV-S 7-mer peptide insert
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL0762 (pQCascade_crRNA-nt)
Plasmid#130636PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
hDAGLa-V5
Plasmid#87676PurposeExpresses human DAGL? protein with intracellular V5 tag located on the C-terminalDepositorInsertHuman Diacylglycerol Lipase Alpha (DAGLA Human)
UseTagsV5 tagExpressionMammalianMutationPromoterT7Available sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-APEX2
Plasmid#109420PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL0828 (pQCascade_crRNA-4)
Plasmid#130637PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD1
Plasmid#195839PurposeExpresses human FZD1 with an N-terminal HiBiTDepositorInsertFZD1 (FZD1 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF051
Plasmid#155328PurposeConstitutive expression of SRDX-EL222 in plantsDepositorInsertPCaMV35S-SRDX-NLS-EL222-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
HSC1-HS4-GiP
Plasmid#58540PurposeDerived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTRDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
Core HS4 insulator sequence (dimer)
UseRetroviralTagsExpressionMammalianMutationPromoterHuman EF1-alphaAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB3045(gRNA XI-3)
Plasmid#73287PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-gpASNase1
Plasmid#121526PurposeExpresses gpASNase1 in mammalian cells.DepositorInsertgpASNase1
UseRetroviralTagsFLAGExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUB6-CDC34-HA (pRKB261)
Plasmid#99145PurposeMammalian expression construct to express HA-tagged CDC34DepositorInsertCDC34 (CDC34 Human)
UseTagsHAExpressionMammalianMutationPromoterpTightAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNIFV
Plasmid#111085PurposepNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectorsDepositorInsertempty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
UseDeleted foamy virusTagsExpressionMutationat nt 2334 A changed to T, at nt 2495 A changed t…PromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SIM)3
Plasmid#127148PurposeMammalian expression plasmid for mCherry-(SIM)3 client to assess SUMO-SIM scaffolds.DepositorInsert3 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Ac-AChBP
Plasmid#63128PurposeExpression of Aplysia californica AChBPDepositorInsertAChBP
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-mKate2.5f
Plasmid#99129PurposeAlso known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoterDepositorInsertmKate2.5f
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP3915
Plasmid#113335PurposeBeta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoterDepositorInsertArtificial Transcription Factor (McIsaac et al, 2013)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Tet-GBP1-C589A
Plasmid#138090PurposeLentiviral backbone for inducible expression of human GBP1 missing the reactive cysteine of the CaaX-box (GBP1-C589A)DepositorInserthuman GBP1 (GBP1 Human)
UseLentiviralTagsExpressionMammalianMutationcysteine 589 to alanine (C589A)Promoterminimal CMV promoter with heptamerized tet‐operat…Available sinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH-mono-MAD7
Plasmid#170134PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertMAD7(ErCas12a)
UseCRISPRTagsExpressionPlantMutationPromotermaize Ubiquitin-1, switchgrass Ubiquitin-1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP
Plasmid#198863PurposePlasmid encoding for 2 gRNAs targeting the human TERT geneDepositorInsertTERT sgRNA (TERT Human)
UseTagsExpressionMammalianMutationPromoteru6Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-mTurquoise2
Plasmid#99115PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mTurquoise2DepositorInsertDIO-mTurquoise2
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-Flag-Mili-EGFP
Plasmid#212576PurposeLentiviral expression of Flag-tagged mouse Mili in mammalian cellsDepositorInsertMili (Piwil2 Mouse)
UseLentiviralTags5x Flag and SNAP tagExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-BtW-eSpCas9
Plasmid#133358PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expressionDepositorInserteSpCas9
UseTagsFlag-tagged eSpCas9ExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCarCBADE_ProB(I69E)A
Plasmid#117756PurposeProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backboneDepositorInsertGlutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
UseTagsExpressionBacterialMutationPromoterPLac(UV5)Available sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLX-Z4 (GB3375)
Plasmid#160648PurposepLX series: RK2-based T-DNA binary vector for plant transformation (alpha1)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAVA-LGF2-Gal11p
Plasmid#127481PurposePositive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interactionDepositorInsertLGF2-Gal11p (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2
Plasmid#127480PurposePositive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interactionDepositorInsertGal11p-LGF2 (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIC-His-RGS4
Plasmid#204847PurposeExpress His tagged-RGS4 in bacterial cells for protein purificationDepositorInsertRGS4 (RGS4 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-ASCL2
Plasmid#133375PurposePiggybac vector for constitutive expressionDepositorInsertASCL2 (ASCL2 Human)
UseTagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK-FLAG-TRAF4
Plasmid#58316PurposeFLAG-tagged mouse TRAF4DepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK-HA-TRAF5
Plasmid#58315PurposeHA-tagged mouse TRAF5DepositorInsertTNF receptor associated factor 5 (Traf5 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-myc
Plasmid#109422PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and mycExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-mRuby2
Plasmid#99117PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2DepositorInsertDIO-mRuby2
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-cTF
Plasmid#87743PurposeIPTG-inducible expression of ligase-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and cTF (chimeric transcription factor base…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
WT C-His Ana GvpC in pET28a
Plasmid#85732PurposeExpresses His-tagged GvpC from Anabaena flos-aquae in E.coliDepositorInsertGas vesicle protein C (his-tagged)
UseTagsHis-tagExpressionBacterialMutationAdded an extra glycine after the methionine at th…PromoterT7Available sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-IGF2
Plasmid#133374PurposePiggybac vector for constitutive expressionDepositorInsertIGF2 (IGF2 Human)
UseTagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
evoCas9
Plasmid#138558PurposeExpresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSDDepositorInsertevoCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationM495V, Y515N, K526E, R661QPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)
Plasmid#99144PurposeInducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139ADepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationFirst 127 codons are optimized to reduce GC conte…PromoterpTightAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG/hArf4(WT)-mCherry
Plasmid#79406PurposeExpresses C-terminally mCherry-tagged Arf4(WT) in mammalian cellsDepositorInsertArf4 (ARF4 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only