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PurposeConstitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMCSCen1
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Vector typeBacterial Expression, CRISPR ; in Y. lipolytica
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCas9
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SpeciesS. pyogenes MGAS5005
- Promoter unknown
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F20 (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namesgRNA
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SpeciesSynthetic
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byHal S.Alper , The University of Texas at Austin,Austin,TX,USA
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAS1yl was a gift from Sheng Yang (Addgene plasmid # 73226 ; http://n2t.net/addgene:73226 ; RRID:Addgene_73226) -
For your References section:
Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system. Gao S, Tong Y, Wen Z, Zhu L, Ge M, Chen D, Jiang Y, Yang S. J Ind Microbiol Biotechnol. 2016 Aug;43(8):1085-93. doi: 10.1007/s10295-016-1789-8. Epub 2016 Jun 27. 10.1007/s10295-016-1789-8 [pii] PubMed 27349768