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Plasmid#192437PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-RSET-iGABASnFR2-6xHis
Plasmid#218885PurposeBacterial expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseTags6xHisExpressionBacterialMutationS99A F102Y F104Y L178SPromoterT7Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM/SAR-CAG-aSyn(A53T)/HA-WPRE-bGHpA
Plasmid#194242PurposeExpresses human alpha-Synuclein A53TDepositorInsertaSynuclein(A53T) (SNCA Human)
UseAAVTagsHAExpressionMutationA53TPromoterSAR-CAGAvailable sinceJan. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Tol2-LyzC-RacFRET-U6ac-ctrl guides
Plasmid#168247Purpose"label active Rac; control for neutrophil-specific knockout"DepositorInsertRacFRET
UseZebrafish expressionTagsExpressionMutationPromoterLyzCAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-UBR5
Plasmid#231238PurposeFlag-tagged wild-type UBR5 over-expression construct for transient expression in mammalian cellsDepositorInsertUBR5 (UBR5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS-G156E
Plasmid#181716PurposeGalactose inducible expression of FUS-G156EDepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationG156EPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-Skp2
Plasmid#19947DepositorInsertS-phase kinase-associated protein 2 (p45) (SKP2 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-NlucP
Plasmid#162595PurposeC-terminally PEST-tagged NanoLuc under TRE3G promoterDepositorInsertNanoLuc-PEST
UseTagsPESTExpressionMammalianMutationPromoterTRE3G promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX-TRV1
Plasmid#180515PurposeAgrobacterium tumefaciens-based expression of TRV1DepositorInsertTobacco rattle virus RNA1
UseTagsExpressionPlantMutationPromoter35S CaMVAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
qTAG-KO-Puro
Plasmid#227262PurposeEmpty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Puro cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1
Plasmid#175049PurposeOverexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNatB (pACYCduet-naa20-naa25)
Plasmid#53613PurposeAllows expression of the fission yeast NatB complex - chloramphenicol markerDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAdx-CMV-iCre-P2A-tdTomato
Plasmid#73351PurposeExpress iCre and tdTomato under CMV promoterDepositorInsertCMV-iCre-tdtomato
UseAdenoviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVP91A-pcaHG
Plasmid#113766PurposeExpresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.DepositorInsertsβ subunit of Protocatechuate 3,4- Dioxygenase
α subunit of Protocatechuate 3,4- Dioxygenase
UseTagsMet-6xHisExpressionBacterialMutationPromoterT5 promoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC1A5
Plasmid#131974PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC1A5 (SLC1A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
HaloTag-bActinCDS-bActinUTR-MS2V5
Plasmid#102718PurposeHalo-Actin reporter for detection of both mRNA and proteinDepositorInsertHaloTag-bActinCDS-bActin3'UTR-MS2V5
UseLentiviralTagsHaloTag and MS2V5ExpressionMutationPromoterUbCAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgRNA(CTRL)-CMV-eGFP
Plasmid#194017PurposeExpresses a gRNA that targets the LacZ gene (serves as control) and eGFPDepositorInsertssgRNA(CTRL)
eGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-PGK-destination
Plasmid#60436PurposeDestination vector with PGK - Hygromycin selection markerDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSynapsin_NLS_GFP_P2A_His6_GluA2
Plasmid#170453PurposeExpresses histidine tagged GluA2 in neuronal cellsDepositorInsertGluA2 (Gria2 Mouse)
UseLentiviralTagsHexa-histidineExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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FAB(ER-mito)
Plasmid#214427PurposeDetection of ER-mito contact siteDepositorInsertFRB-V5
UseTagsCFAST10-Halo and RspAN-FRB-V5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-STAT3
Plasmid#111934PurposeExpresses human STAT3 fused to GFP in mammalian cellsDepositorInsertSignal transducer and activator of transcription 3 (STAT3 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-SpCas9-NG
Plasmid#117919PurposeExpresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.DepositorInsertSpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC30A6
Plasmid#132065PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC30A6 (SLC30A6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pB-Neo-Mito-DsRed
Plasmid#210328PurposeOverexpression of Mito-DsRedDepositorInsertMito-DsRed
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0801
Plasmid#141707PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertATF3 (ATF3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
modRNAc0-ABE8e-P2A-GFP
Plasmid#178177PurposeIVT template plasmid for making ABE8e-P2A-GFP modRNADepositorInsertABE8e
UseIvt template plasmidTagsP2A-GFPExpressionMutationPromoterT7 promoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
mu-IFNg-pCIneo
Plasmid#163517PurposeExpresses murine IFN gamma in mammalian cellsDepositorInsertmurine-IFN gamma (Ifng Mouse)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pL99
Plasmid#78577PurposeOver-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR systemDepositorTypeEmpty backboneUseStreptomyces expressionTagsExpressionBacterialMutationPromoterinducible PnitA-NitR systemAvailable sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG
Plasmid#63169PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporterDepositorInsert"spaghetti monster GFP-FLAG"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-mir-RUP-PheS
Plasmid#44011PurposeLentiviral shRNA vector (pGIPz based) for inducible knockdownDepositorInsertPheS Gly294
UseLentiviralTagsRFPExpressionMammalianMutationPromoterTRE2Available sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAB150
Plasmid#101661PurposeOpto-T7RNAP*(563)DepositorInsertOpto-T7RNAP*(563)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETMCSI-T4P_Y320A
Plasmid#122522PurposeExpression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesisDepositorInsertgp43 - DNA polymerase (43 Escherichia virus T4)
UseTagsExpressionBacterialMutationtyrosine 320 changed to alaninePromoterT7 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-WT-VWF
Plasmid#124794PurposeExpresses multimeric human VWF with C-terminal c-Myc tagDepositorInsertVWF (VWF Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGK-HELLO LV
Plasmid#202001PurposeExpresses HELLO neoantigen in tumor cellsDepositorInsertHELLO
UseLentiviralTagsFLAGExpressionMutationPromoterpGKAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKJ0463
Plasmid#228715PurposeBacterial expression of epT7 RNAP; for protein production and purificationDepositorArticleInsertSumo_epT7
UseTagsExpressionBacterialMutationT3M/G47A/E643GPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(S473D)
Plasmid#86577PurposeExpresses cleavable human full-length Akt1(S473D) phosphomimeticDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130; S473D phos…PromoterAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fucci(CA) hCdt1-iRFP hGeminin-iRFP
Plasmid#190182PurposeFluorescent reporter vector for visualization of G0 and other cell cycle phases.DepositorInsertsUseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only