pGP-RSET-iGABASnFR2-6xHis
(Plasmid
#218885)
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PurposeBacterial expression of improved GABA sensor (positive change in fluorescence)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218885 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSET
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Backbone manufacturerThermoFisher Invitrogen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiGABASnFR2
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Alt namevariant 4409
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SpeciesPseudomonas fluorescens, A. victoria (jellyfish)
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Insert Size (bp)1710
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MutationS99A F102Y F104Y L178S
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGP-RSET-iGABASnFR2-6xHis was a gift from GENIE Project (Addgene plasmid # 218885 ; http://n2t.net/addgene:218885 ; RRID:Addgene_218885)