pDisplay-Gncp-iGluSnFR
(Plasmid
#107337)
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PurposeNoncircularly permutated (ncp) variant of iGluSnFR. Membrane-displayed.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 107337 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDisplay
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5268
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Modifications to backbone6918
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGltI based and noncircularly permutated glutamate biosensor
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Alt nameGncp-iGluSnFR
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SpeciesSynthetic
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Insert Size (bp)1650
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that mutation M178K in the Neomycin resistance cassette was found during Addgene's quality control. Neomycin selection has not been tested for this plasmid, but should not affect sensor function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDisplay-Gncp-iGluSnFR was a gift from Robert Campbell (Addgene plasmid # 107337 ; http://n2t.net/addgene:107337 ; RRID:Addgene_107337) -
For your References section:
Genetically Encoded Glutamate Indicators with Altered Color and Topology. Wu J, Abdelfattah AS, Zhou H, Ruangkittisakul A, Qian Y, Ballanyi K, Campbell RE. ACS Chem Biol. 2018 Jan 22. doi: 10.1021/acschembio.7b01085. 10.1021/acschembio.7b01085 PubMed 29308878