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Plasmid#70145Purposemammalian expression of MKLP1 with an eGFP fusionDepositorInsertMKLP1 (KIF23 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ntn1-Fc-His
Plasmid#72104PurposeExpresses the entire Netrin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNtn1 (Ntn1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-NeoR-CAG-EGFPnls-WPRE
Plasmid#191692PurposeFor knocking pCAG-EGFPnls into AAVS1 locusDepositorInsertAAVS1 homology arms and pCAG-EGFPnls
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-WT-STAT1
Plasmid#71454PurposeLentiviral expression of WT-STAT1DepositorInsertSTAT1 (STAT1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSII-EF/mt-(n1)StayGold
Plasmid#185823PurposeA soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.DepositorInsertmt-(n1)StayGold
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAIP hIL4 co
Plasmid#74169PurposeLentiviral vector for expression of human codon optimized IL4 with puromycin resistance.DepositorInsertIL4 (IL4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mTagBFP2-WPRE
Plasmid#193339PurposeAAV-mediated expression of mTagBFP2 under the TRE promoter in mammalian cellsDepositorInsertmTagBFP2
UseAAVTagsExpressionMammalianMutationA XhoI site in the coding sequence is silencedPromoterTREAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-HRas S17N
Plasmid#18665DepositorInsertH-Ras (HRAS Human)
UseTagsmonomeric EGFPExpressionMammalianMutationS17N mutation in H-RasPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-pCMV-PfV-GS-Sapphire
Plasmid#116933PurposeMammalian vector for expression of the 40nm GEMDepositorInsertPfv
UseTagsSapphireExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On shYAP1-9
Plasmid#193668PurposeTet inducible knockdown of YAP1DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA
Plasmid#37120PurposeDO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cellsDepositorInsertTdTomato-EGFP
UseAAV and Cre/Lox; Cre-switchTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Flag-c-Myc
Plasmid#102626PurposeExpresses c-Myc in mammalian cellsDepositorInsertMYC proto-oncogene, bHLH transcription factor (MYC Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hChATp-tdTomato
Plasmid#197564PurposeExpresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragmentDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterhuman ChAT promoterAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHLuc (Antares-SEPLuc C1A4E+6)
Plasmid#141089Purposefinal optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosisDepositorInsertAntares-T2A-puromycin-IRESv24-SEP-C1A4E+6
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM60 FLAG-RAB35 wt
Plasmid#107104PurposeLentiviral vector that codes for FLAG-RAB35 wildtype (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.DepositorInsertRAB35 (RAB35 Human)
UseLentiviralTagsFLAG tagExpressionMammalianMutationwildtypePromoterCMVAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.3b
Plasmid#111056PurposeExpresses dLight1.3b in mammalian cellsDepositorInsertdLight1.3b
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-GFP
Plasmid#159654PurposeEmpty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-EGFP-WPREpA
Plasmid#84469PurposepAAV-RAM-EGFPDepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterTREAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10
Plasmid#196079PurposeConstitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.DepositorInsertgRNA targeting Adam10
UseCRISPRTagsExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10
Plasmid#196085PurposeInducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.DepositorInsertgRNA targeting Adam10
UseCRISPRTagsExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-GFP
Plasmid#80491Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expressionDepositorInsertCAG-GFP
UseDonor vector (human)TagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM60 FLAG-RAB35 S22N
Plasmid#107106PurposeLentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.DepositorInsertRAB35 (RAB35 Human)
UseLentiviralTagsFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.GCaMP6f.WPRE.SV40
Plasmid#100835PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCAGAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_Capture1_APOBEC_HA_P2A_mRuby
Plasmid#183901PurposeInducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTADepositorTypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…TagsCapture1_APOBEC_HA_P2A_mRubyExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-NpHR-EYFP
Plasmid#171621PurposeTRE-NpHR-EYFP reporterDepositorInsertTRE-NpHR-EYFP
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterTetOAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry
Plasmid#159655PurposeEmpty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHEN2 F8-2
Plasmid#186033PurposeExpress VHH F8-2 with 3 c-myc tag in E. coli periplasmDepositorInsertVHH F8-2
UseTags6His and MycExpressionBacterialMutationPromoterlacAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.GCaMP6f.WPRE.SV40
Plasmid#100836PurposeAAV expression of ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1, AAV2, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCAGAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ai34
Plasmid#34881DepositorInsertCAG-Floxed Synaptophysin-tdTomato
UseMouse TargetingTagstdTomatoExpressionMutationPromoterCAGAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_Ado1.0med
Plasmid#153289PurposeExpress the medium-affinity adenosine sensor GRAB_Ado1.0med in astrocytesDepositorInsertAdenosine sensor GRAB_Ado1.0med (medium affinity)
UseAAVTagsExpressionMutationPromoterGfaABC1DAvailable sinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pXL010-Wnt dual (GFP-Fire) reporter
Plasmid#40588DepositorInsertWnt reporter sequences
UseLentiviralTagsExpressionMammalianMutationPromoterWnt promoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-LC3-YFP
Plasmid#99571PurposeLentiviral expression of YFP-tagged LC3DepositorInsertLC3 (MAP1LC3B Human)
UseLentiviralTagsYFPExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
2xFKBP-mCh-KDEL
Plasmid#173010PurposeFKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.DepositorInsert2xFKBP-mCh-KDEL
UseTagsmCherryExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUbC-OsTIR1-myc-IRES-scFv-sfGFP
Plasmid#84563PurposeBicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFPDepositorInsertTransport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
UseLentiviralTagsmyc tag (9x)ExpressionMutationPromoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV PGK1-3xHA
Plasmid#220913PurposeExpresses PGK1-3xHADepositorInsertPGK1-3xHA (PGK1 Human)
UseAffinity Reagent/ AntibodyTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6s.WPRE.SV40
Plasmid#100843PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVTagsExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAT-PB-CD2APtk
Plasmid#86004Purposehomology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassetteDepositorInsertDsRedEx_T2A_puro_dTK
UsePiggybac exciseableTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-ChR2-YFP
Plasmid#171622PurposeTRE-ChR2-YFP reporterDepositorInsertTRE-ChR2-YFP
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterTetOAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PfV-Sapphire-IRES-DSRed
Plasmid#116934PurposeMammalian lenti vector for expression of the 40nm GEM and DSRedDepositorInsertPfv
UseLentiviralTagsSapphireExpressionMutationPromoterCMVAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICE-FLAG-NAT10-siR-WT
Plasmid#59365PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInserthuman NAT10 (NAT10 Human)
UseTagsFLAGExpressionMammalianMutationMutations to render cDNA resistant to a siRNAPromoterCMV-tetAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJR019
Plasmid#198834PurposeInactivation of G protein subunits through Pertussis toxin (PTX)DepositorInsert15xUAS::PTX-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMCherry F8-2
Plasmid#186034PurposeExpress VHH F8-2 as a mCherry fusion in mammalian cellsDepositorInsertVHH F8-2
UseTagsmCherryExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAIP hIL2 co
Plasmid#90513PurposeLentiviral vector for expression of human codon optimized IL2 with puromycin resistance.DepositorInsertIL2 (IL2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6f.WPRE.SV40
Plasmid#100837PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
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His-GFP-FCHo2
Plasmid#170870PurposeMammalian expresssion of His-GFP-FCHo2.DepositorInsertFCH domain only 2 (Fcho2) (Fcho2 Mouse)
UseTags(His)6 and mEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA2-DDB1
Plasmid#19909DepositorInsertDamage-specific DNA binding protein 1 (DDB1 Human)
UseTagsHAExpressionMammalianMutationplease see depositor comment belowPromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA
Plasmid#63931PurposeEmpty AAV-RAM plasmid w. multiple cloning site (MCS)DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterpRAM and TREAvailable sinceDec. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-COX4-dAPEX2
Plasmid#117176PurposePlasmid name in publication: pAAV-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertCOX4-dAPEX2
UseAAVTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only