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pAAV-hSyn-ConVERGD-ConFoff-eGFP-W3SL

Plasmid

#218751

Purpose

Provides NOT intersectional (Cre NOT Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEF6.mCherry-TSG101

Plasmid

#38318

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

TSG101 (TSG101 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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HA-NFAT1(4-460)-GFP

Plasmid

#11107

Purpose

Expresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells

Depositor

Anjana Rao

Article

Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

Insert

NFAT1 (Nfatc2 Mouse)

Use

Tags

3x HA and GFP

Expression

Mammalian

Mutation

Contains amino acids 4-460 of NFAT1; L78P compare…

Promoter

CMV

Available since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

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gRNA_AAVS1-T2

Plasmid

#41818

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-G-Flamp1

Plasmid

#188567

Purpose

green biosensor for cAMP in mammalian cells

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1

Use

Tags

His tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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rescue plasmid

Plasmid

#135971

Purpose

expresses copGFP and puromycin resistance genes

Depositor

Yongming Wang

Article

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

Plasmid

#208942

Purpose

The CE1 construct, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-Mango II x 24

Plasmid

#127587

Purpose

Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

Depositor

David Rueda

Article

Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

Insert

mCherry-Mango II x24

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

pTRE Tet ON

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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MyrPalm-CKAR

Plasmid

#14862

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Use

Tags

CFP, Myr/Palm, and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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GALT-SEP-mRuby3

Plasmid

#200938

Purpose

trans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Inserts

GalT (B4GALT1 Human)
SEP-mRuby3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCk4_spacer

Plasmid

#136074

Purpose

Use when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-LacI

Plasmid

#183920

Purpose

Lac repressor labeled with GFP; can be used to label lacO sites

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

GFP-LacI (lacI Synthetic)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

SV2

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pShuttle CMV RGECO-TnT

Plasmid

#124643

Purpose

Myofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination.

Depositor

Paul Robinson, Hugh Watkins, Matthew Daniels

Article

Sparrow et al Circ Res. 2019 Feb 8. doi: 10.1161/CIRCR

Insert

RGECO-TnT (TNNT2 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET-MagLOV

Plasmid

#219957

Purpose

Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

Depositor

Andrew York

Article

Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

Insert

MagLOV

Use

Tags

6xHis

Expression

Bacterial

Mutation

AsLOV2 C450P L496V Q513K G528K D540M

Promoter

T7

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-GSK3β-#2

Plasmid

#32497

Depositor

Alex Toker

Article

Yoeli-Lerner et al Mol Cancer Res. 2009 Mar;7(3):425-32. Ep

Insert

GSK3B (GSK3B Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only
SauKKHCas9 PE2*

Plasmid

#169852

Purpose

Mammalian Expression, sauCas9-KKH based prime editor

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SaCas9KKH-N580A-M-MLV

Use

Tags

bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

Expression

Mammalian

Mutation

Promoter

cmv

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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Lys-eMagB-iRFP

Plasmid

#162249

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

p18-eMagB-iRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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3TERM_Nos-35S

Plasmid

#136116

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_Nos-35S (A. tumefaciens, CaMV)

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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mEmerald-H3-23

Plasmid

#54115

Purpose

Localization: Nucleus/Histones, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

H3 (H3C2 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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KO plasmid

Plasmid

#135970

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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