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pLenti6.3-sRGN3.1-BlastR

Plasmid

#220964

Purpose

Expresses human codon-optimized sRGN3.1

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

sRGN3.1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pET His6 Thioredoxin TEV co-transformation cloning vector (13K-T)

Plasmid

#48322

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and Thioredoxin

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-ecfp

Plasmid

#65030

Purpose

GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

ecfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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UKNP1.11.6

Plasmid

#97382

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB516

Plasmid

#74858

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mRuby2-MACROD1

Plasmid

#172587

Purpose

For transient expression of C-terminal mRuby2-tagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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OA984

Plasmid

#120363

Purpose

Expresses an single chain antibody 1C19 that targets all four DENV serotypes and expresses a red fluorescent protein reporter

Depositor

Omar Akbari

Article

Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.

Insert

1C19 single chain antibody

Use

Unspecified

Tags

Expression

Mutation

Promoter

AAEL010782 carboxypeptidase A (CPA) gene Promoter

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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p6610 MSCV-IP N-HAonly TIMP1

Plasmid

#35009

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

TIMP1 (TIMP1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
N-Myc-53BP1 D1521A pLPC-Puro

Plasmid

#19837

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Oct 19. ():.

Insert

p53 Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

changed Asp1521 to Ala

Promoter

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKII(0.3)-mVenus-FKBP-K7GEF

Plasmid

#225828

Purpose

A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

Depositor

Haruo Kasai

Article

Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

Insert

mVenus-FKBP-K7GEF

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

Plasmid

#204816

Purpose

Bacterial expresison of Enterovirus A71 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

EV A71 3C protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBABE-hygro-2xMyc-Rac1-WT

Plasmid

#128580

Purpose

For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-pBAD

Plasmid

#54844

Purpose

Localization: Protein Expression Vector , Excitation: 434, Emission: 474

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

6x His, T7 tag, and TEV site

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pEK36

Plasmid

#210598

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter

Use

Tags

mClover3

Expression

Bacterial and Yeast

Mutation

Promoter

SpH2Bpro

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAG305GPD-ccdB

Plasmid

#14138

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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acta2_GFP_pA_pDestTolpA2

Plasmid

#78684

Purpose

acta2:GFP transgenesis construct

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

acta2

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; HE1A:BFP -1

Plasmid

#180008

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; HE1A: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-gp78c/2767

Plasmid

#11429

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78c (AMFR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

n/a

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pBT128c

Plasmid

#138512

Purpose

P2

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

inteinC-dSpCas9

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-mHSF1-zeo

Plasmid

#17755

Depositor

Susan Lindquist

Article

Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

Insert

HSF1 (Hsf1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only