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Plasmid#164410PurposeRetroviral expression plasmid of sgRNA with mCherryDepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only
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TFORF1109
Plasmid#143466PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCREBBP (CREBBP Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC52A3
Plasmid#132181PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC52A3 (C20orf54 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLEX307-TMPRSS2-puro
Plasmid#158457PurposeConstitutive lentiviral expression of human TMPRSS2.DepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pmec-18 caspase-3 (p12)::nz [TU#813]
Plasmid#16082DepositorInsertcaspase-3 (p12) :: nz (CASP3 Human)
UseTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_lbMicroID2-HA
Plasmid#188522PurposeEncodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cellsDepositorInsertlbMicroID2
UseTransposon mediated gene integrationTagsHAExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-STAT6-IRES-Neo
Plasmid#35482DepositorInsertSTAT6 (STAT6 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA
Plasmid#108748PurposeExpresses aa28 to C-terminus of Flp recombinase fused to GAI CIDDepositorInsertFlp recombinase fragment aa28 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-CDK13_IDG-K
Plasmid#135276PurposeGateway destination clone of CDK13 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-CDK13 (CDK13 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
PIEZO1-HaloTag
Plasmid#207834PurposePIEZO1 fusion for biochemistry and live imagingDepositorInsertPIEZO1 (PIEZO1 Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_nV5-AMOT (p130)
Plasmid#172997Purposeconstitutive expression of V5-tagged AMOT (p130)DepositorInsertV5-AMOT (p130) (AMOT Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMH0006
Plasmid#135448PurposeHuman expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.DepositorInsertdCas9-BFP-KRAB
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
WT GBA pcDNA3.1
Plasmid#188580PurposeEctopic expression of wild-type GBADepositorInsertGBA (GBA1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
915-Plakophilin 2a-Flag
Plasmid#32230DepositorInsertPlakophilin 2a (PKP2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNTI934
Plasmid#216637PurposeCal-ID-PM (V5) Plasma membrane-localized Cal-ID, mammalian expression and lentivirus productionDepositorArticleInsertCal-ID-PM
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_puro-mKate-lox2272
Plasmid#162076Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya
Plasmid#186980Purposemammalian expression of Cluc-Gluc reporterDepositorInsertCluc, Gluc
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH2B-miRFP670nano3
Plasmid#184670PurposeHistone 2B labelled with near-infrared fluorescent protein miRFP670nano3DepositorInsertH2B-miRFP670nano3
UseTagsmiRFP670nano3ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2 sgNRF2-3
Plasmid#186838Purposeknock out NRF2 in mammalian cellsDepositorInsertNrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)
Plasmid#42335PurposeA human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.DepositorArticleHas ServiceCloning Grade DNAInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationPromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only